摘要
近年来有研究显示胡桃醌对细菌具有一定的抑制作用,但其对细菌的抑菌机理尚不明确。文章从胡桃醌对大肠杆菌呼吸和能量代谢的影响来揭示其抑菌机理。分别对培养基中溶氧量、葡萄糖含量、细胞内ATP含量及ATP酶活性进行测定。结果表明:在最低抑菌浓度(MIC)下,培养基中的溶氧量略微下降,8h后趋于恒定,溶氧量共下降了24.2%,而乙醇对照(CK2)下降了39.4%;培养基中的葡萄糖含量先下降后上升,葡萄糖含量增加9.1%,而CK2则下降6.4%;菌体内的ATP含量先是剧烈下降,然后缓慢上升,4h后趋于恒定,MIC时下降最少;ATP酶活性起先略有下降,随后上升,MIC时上升的最少,而后又略有下降,显示出一定的波动性。以上结果说明随着时间增大,不同浓度的胡桃醌对大肠杆菌的呼吸和能量代谢均有一定影响。
In recent years,studies have found that juglone shows inhibition of bacteria,but its antibacterial mechanism is unclear.This article reveals juglone＇s antibacterial mechanism in terms of Escherichia coli respiration and the energy metabolism.The medium on oxygen,glucose content,cells of ATP content and ATP enzyme activity.The results showed that the bacteriostatic on the lowest concentration（MIC）,liquid medium of oxygen to slightly decreased,respectively.After eight hours tended to constant,the content of dissolved oxygen fell 24.2%,while,the ethanol contrast（CK2） fell 39.4%.The medium of the glucose levels dropped at first and then rose,the glucose content rose 9.1%,while CK2 dropped 6.4%.While Escherichia coli ATP content first steeped descent,then slow rose,4 hours after tented to constant,MIC fell at least. ATP enzyme activity declined slightly at the beginning of experiment,then increased slightly,MIC rose at least,and finally dropped which seemed to be fluctuant.Testify that as increasing of the time,different concentrations of juglone to breathing and energy metabolization of Escherichia coli had certain effect.
出处
《食品与机械》
CSCD
北大核心
2011年第6期131-134,共4页
Food and Machinery
基金
辽宁省科学技术基金项目(编号:20102195)
关键词
胡桃醌
大肠杆菌
呼吸
能量代谢
juglone
Escherichia coli
breath
energy metabolization
