摘要
本研究在测定不同球孢白僵菌Beauveria bassiana和金龟子绿僵菌Metarhizium anisopliae菌株的生长速率与产孢量的基础上,采用孢悬液浸渍法进行了对油茶新害虫——黑足角胸叶甲Basilepta melanopus成虫的生物测定,旨在筛选出感染该成虫的高致病力菌株,为防治该虫提供新的生物资源。结果表明:不同菌株生长速率和产孢量间存在显著差异,MaYTTR-04,BbFZ-17,MaZPTR-01和BbTK-01生长速率和产孢量均显著高于其他菌株。接种后,叶甲成虫累积死亡率随时间的增加而逐渐增高,接种白僵菌7d后,成虫校正死亡率全部达到100%;接种MaZPTR-01和MaYTTR-04两个绿僵菌菌株14d后,成虫死亡率分别为80.3%和78.8%。而且接种白僵菌后,叶甲成虫的僵虫率显著较绿僵菌高,尤其以BbTK-01和BbFZ-17两个菌株较好,分别达到85.7%和75.8%。BbXJ-01,BbFZ-17和BbTK-013个白僵菌菌株的LT50最小,分别为3.0,3.3和3.4d;MaYTTR-04和MaZPTR-01两个绿僵菌的LT50分别为6.0和6.2d。结果说明,白僵菌对叶甲成虫的致病力较强,尤其是BbTK-01和BbFZ-17两个菌株,不仅致死率高,且致死速度快,僵虫率高,同时这2个菌株生长速度快、产孢量大,具有优良的生产特性,在黑足角胸叶甲的生物防治中将有重要的应用价值。
In order to screen entomopathogenic fungi strains with high virulence against the new pest Basilepta melanopus on Camellia oleifera,the growth rate and sporulation of different Beauveria bassiana and Metarhizium anisopliae strains were investigated,and bioassay evaluation of B.bassiana and M.anisopliae against B.melanopus adults was carried out through dipping in spore suspension.The results showed that the growth rate and sporulation of different fungi strains were significantly different.The four strains,i.e.,MaYTTR-04,BbFZ-17,MaZPTR-01 and BbTK-01,had higher growth rate and sporulation than other strains.The cumulative mortality of B.melanopus adults was gradually increased with time after inoculation with B.bassiana and M.anisopliae.The cumulative mortality of B.melanopus adults reached 100% at 7 d after inoculation with B.bassiana,while the mortality of adults at 14 d after inoculation with MaZPTR-01 and MaYTTR-04 were 80.3% and 78.8%,respectively.The cadaver rate of adults inoculated with B.bassiana was significantly higher than that inoculated with M.anisopliae.The cadaver rates of BbTK-01 and BbFZ-17 were highest,being 85.7% and 75.8%,respectively.The median lethal time（LT50） values of B.bassiana strains BbXJ-01,BbFZ-17 and BbTK-01 were shortest,being 3.0,3.3 and 3.4 d,respectively.However,the LT50values of the two M.anisopliae strains,MaZPTR-01 and MaYTTR-04,were 6.0 d and 6.2 d,respectively.The results of bioassay showed that B.bassiana strains had higher virulence to B.melanopus adults than M.anisopliae,especially the two strains,BbTK-01 and BbFZ-17,had higher lethality and infection rate than other strains,and at the same time,they had the characteristics of rapid growth and high sporulation,suggesting that the two strains have great potential in biocontrol of B.melanopus adults.
出处
《昆虫学报》
CAS
CSCD
北大核心
2011年第11期1281-1287,共7页
Acta Entomologica Sinica
基金
福建省科技厅农业科技重点项目(2011N0011)
福建省林业厅科研项目(闽林科2009[8])
科技部农业科技成果转化资金项目(2010GB2C400215)
福建省森林培育与林产品加工重点实验室资助项目
