摘要
目的:孔石莼中含有4种相对分子量的水溶性多糖(151.7、64.5、58.0ku和28.2ku)。了解不同相对分子量段孔石莼多糖对辐射诱发小鼠骨髓细胞微核形成的抑制作用及效果比较。方法:将通过膜分离纯化得到的3种不同相对分子量段的孔石莼多糖(≥100ku、30～100ku及≤30ku)分别给相应设置的3个实验组小鼠进行灌服,另设辐照对照组和空白对照组;辐照后制备各组动物的骨髓涂片,计数多染性红细胞的微核发生率。结果:低相对分子量段的孔石莼多糖对辐射诱发的小鼠骨髓细胞微核具有一定的抑制效应,其中≤30ku相对分子量的孔石莼多糖对其造成的抑制作用最为显著(P<0.01)。结论:低相对分子量孔石莼多糖对辐射引起小鼠染色体损伤具有一定的抑制作用,由此推断低相对分子量孔石莼多糖具有一定的抗辐射作用。
Objective：There are four kinds of molecular weight water-soluble polysaccharide in Ulva pertusa,including 151.7 ku,64.5 ku,58.0 ku and 28.2 ku.In this study,to explore the role of the different molecular weight polysaccharide from Ulva pertusa on antagonizing the radiationinduced micronuclei formation of mice bone marrow cells.Methods：Three different molecular weight polysaccharides（≥100 ku,30～100 ku and≥30 ku） from Ulva pertusa who isolated by membrane injected mice by gastric perfusion.A positive and a negative groups were set as contrast groups.Bone marrow smears were sampled after Irradiated for calculating the micronuclei rate of polychromatic erythrocytes.Results：Low molecular weight polysaccharides from Ulva pertusa on the radiation-induced micronuclei in mouse bone marrow cells showed some inhibitory effect,which≤30 ku Ulva polysaccharides caused by inhibition of its most significant（P0.01）.Conclusion：It is suggested that the Low molecular weight polysaccharides from Ulva pertusa exert some protective effect to chromosomes in mammalian like mice,according to the outcome of an obviously inhibitory effect of its extraction on micronuclei formation induced by radiation.
出处
《食品科技》
CAS
北大核心
2011年第12期196-198,共3页
Food Science and Technology
基金
总装备部921应用系统生命分系统子课题
哈尔滨工业大学985学科后备带头人培育计划
关键词
孔石莼
多糖
辐射
骨髓微核
Ulva pertusa Kjellm
polysaccharide
irradiation
bone marrow micronuclei
