摘要
目的探索应用骺板组织工程治疗骺板损伤的新方法。方法将骺板软骨细胞与可降解多孔丝素材料复合，植入骺板损伤的动物模型中，同时设单纯植人可降解多孔丝素材料组和空白对照组。应用组织学Masson三色染色、细胞学BrdU细胞示踪技术、影像学X线检测等方法，观察骺板修复、短缩畸形和成角畸形情况。结果通过Masson三色染色我们发现：骺板软骨细胞复合可降解多孔丝素材料组（SF＋cell）存在一定的短缩畸形和成角畸形，骺板损伤处发现类似正常的柱状排列细胞；单纯植入可降解多孔丝素材料组（SF）存在明显的短缩畸形和成角畸形，骺板损伤处发现松散的柱状排列细胞；空白对照组存在显著的短缩畸形和成角畸形，骺板损伤处未见柱状排列细胞。通过X线检测我们发现：三组短缩畸形分别为（3．24±0．58）％、（6．32±0．37）％和（8．47±0．51）％。三组成角畸形分别为（4．89±1．27）°、（10．67±1．58）。和（18．44±2．65）°。其短缩畸形和成角畸形的程度均存在统计学意义（均P=0．0001，P〈0．01）。结论骺板组织工程方法治疗骺板损伤是一种可行的方法，骺板软骨细胞复合可降解多孔丝素材料组的疗效优于单纯植人可降解多孔丝素材料组。
Objective To explore the＇effect of tissue engineered epiphyseal plate on epiphyseal plate injury. Methods The engineered epiphyseal plate and scaffold were respectively implanted to the animal model of epiphyseal plate injury, Masson staininng and BrdU tracing assay, as well X-ray were utilized for evaluation of efficacy. Results The Masson staining indicated reduced deformity degree in engineered epiphyseal plate group compared with scaffold group, whereas the deformity degree in scaffold group was less in contrast to the control group. The X-ray results showed that in engineered epiphyseal plate group, scaffold group and control, the shorten deformity were respectively 3. 24 ± 0. 58, 6. 32 ± 0. 37 and 8. 47 ± 0. 51, while the varus deformity for them were 4. 89 ± 1.27, 10. 67 ± 1.58 and 18. 44 ± 2. 65. The difference among them was statistically significant （P = 0. 0001 ）. Conclusions The engineered epiphyseal plate is a better and promising biomaterial to treat epiphyseal plate injury.
出处
《中华小儿外科杂志》
CSCD
北大核心
2011年第12期933-936,共4页
Chinese Journal of Pediatric Surgery
关键词
生长面
组织工程
Growth plate
Tissue engineering
