摘要
背景:可降解的壳聚糖膜高分子生物材料已在防止组织粘连中广泛应用,但由于眼眶空间较小,结构精细,目前尚未广泛应用。目的:探讨壳聚糖膜在防治眼眶损伤后软组织粘连中的作用与机制。方法:普通家兔10只随机数字表法分为壳聚糖膜组和对照组,建立成年家兔眼眶软组织粘连模型,壳聚糖膜组受损上直肌与结膜和相应骨膜之间置入15mm×15mm大小壳聚糖膜防粘连;对照组在损伤面间不用壳聚糖膜。结果与结论:在采用防粘连措施前,两组粘连形成情况像似,4周后,壳聚糖膜组粘连情况较对照组减轻(P<0.01)。壳聚糖膜组单位面积的成纤维细胞数量、胶原纤维吸光度值显著低于对照组(P<0.01);胶原纤维的相对面积壳聚糖膜组低于对照组(P<0.01)。两组家兔眼视网膜与视神经未发现明显变化。说明局部植入壳聚糖膜可以抑制成纤维细胞生长,减少胶原纤维的合成,防治眼眶损伤后软组织粘连,且对视网膜与视神经无毒副作用。
BACKGROUND： The macromolecular biomaterial, chitosan membrane （CM） with low immunogenicity, can be resolved and generally used in the field of soft tissue adhesion. But the orbit included much subtle texture and owned the minor space, the application of CM in the orbit has not been general at present. OBJECTIVE： To observe the influence and mechanism of CM in prevention and treatment of soft tissue adhesion after orbital trauma. METHODS： A total of 10 rabbits with 20 orbits were randomly divided into two groups, namely, CM group and control group. The adhesion models of rabbits were established first, then the adhesion tissues were separated after two weeks, and CM about 15 mm×15 mm were implanted between the lesion and periost. The CM was not used in the control group. RESULTS AND CONCLUSION： Four weeks after experiment, the adhesion degree was lower in the CM group than the control group （P 0.01）. The quantity of fibroblasts in unit area and absorbance value in unit area of collagen fibers were both significantly lower in the CM group than the control group （P 0.01）. Relative area of collagen fibers was also lower in the CM group as compared with the control group （P 0.01）. The structures of retina and optic nerve were not changed in histopathology. CM can inhibit the proliferation of fibroblasts, and decrease the synthesis of collagen fibers, which is effective on preventing adhesion after the orbital trauma. And it has no effect to the retina and optic nerve.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第47期8825-8828,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
天津市卫生局科技基金(05ky28)
课题名称"眼眶骨折术后粘连防治的实验与临床研究"~~
