摘要
背景:肿瘤干细胞学说认为,肿瘤干细胞是肿瘤不断增殖的根源,并与肿瘤的浸润转移、耐药现象密切相关。目的:在单细胞水平研究体外传代培养的MCF7细胞中肿瘤干细胞含量变化规律,认识肿瘤干细胞在体外培养环境下的增殖特点。方法:利用单细胞分离种植-成瘤性克隆方法对传代培养后不同时间点MCF7细胞中肿瘤干细胞的比例连续检测,流式细胞仪同步检测相应细胞样本中CD44+CD24-/low亚群的含量变化。结果与结论:传代后培养的MCF7细胞在不同时间点其肿瘤干细胞比例及CD44+/CD24-/low亚群比例均呈现有规律的变化。但CD44+/CD24-/low亚群变化更为显著(36.84%到81.95%),而肿瘤干细胞含量仅在小范围波动(38.54%～47.39%)。结果提示传代培养的MCF7细胞中,肿瘤干细胞具有通过调节自身增殖来保持其比例相对稳定的特点;CD44+/CD24-/low亚群并不代表或者富集MCF7细胞株中的肿瘤干细胞亚群。
BACKGROUND：The hypothesis of tumor stem cells posited that tumor stem cells are the root of tumor proliferation constantly and closely related to tumor biological behaviors such as infiltration,metastasis and drug-resistant.OBJECTIVE：To investigate the proliferation characteristics of tumor stem cells in MCF7 cell line in vitro by studying the tumorigenic-colony forming rate at single cell level.METHODS：Tumor stem cells proportion in MCF7 cell line was detected at series timing after passaged culture by single-cell-transplanting and tumorigenic-colony forming assay.The proportion of the CD44＋/CD24-/low subset was detected by flow cytometry at the same time.RESULTS AND CONCLUSION：The proportion of tumor stem cells and the CD44＋/CD24-/low subset in MCF7cell line both varied regularly after passaged culture.But the variation of CD44＋/CD24-/low（from 36.84% to 81.95%） subset was more drastic than that of tumor stem cells proportion,which only fluctuated in a limited range from 38.54% to 47.39%.The proportion of tumor stem cells was steady in MCF7 cell line after passaged culture,owing to the property of tumor stem cells to modulate and balance the proportion by itself.CD44＋/CD24-/low subset neither represented nor enriched tumor stem cells subset in MCF7 cell line.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第45期8404-8407,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广西研究生教育创新计划资助项目(2008105981002D33)~~
