摘要
目的：建立复方利福平片中异烟肼和吡嗪酰胺的含量测定方法。方法：毛细管气相色谱法，以乙酰苯胺为内标，色谱柱为弹性石英毛细管柱２５ｍ ×０ ．３２ｍ ｍ ×０ ．５２μｍ （ＤＢ１） ；柱温１７０ ℃；气化室温度２５０ ℃；检测器温度２５０ ℃；载气为高纯氮。结果：异烟肼和吡嗪酰胺的线性范围分别是０ ．４２６ ～２ ．１３ｍｇｍｌ 和１ ．２３ ～６ ．１３ｍｇｍｌ；平均回收率分别为９９ ．８１ ％ 和９９ ．６３ ％ 。结论：方法简便，快速，准确，可作为该制剂的检测方法。
OBJECTIVE:To determine isoniazid and pyrazinamide in tablet of Lifuping compound.METHOD:A capillary gas chromatography was employed.The internal stardand reference was acetanilide.Operating conditions were:injector temperature 250℃,detector temperature 250℃,column temperature 170℃,FID detector.Fused silica columns with dimension of 25m×0.32mm ID was coated with chemically bonded SE 30.RESULTS:The calibration equations were linear in the range of 0.426～2.13mg/ml for isoniazid and 1.23～6.13mg/ml for pyrazinamide.The average recoveries were 99.81% for isoniazid and 99.63% for pyrazinamide.CONCLUSION:This method was found to be simple,quick and accurated for the determination of two constituents in tablet of Lifuping compound.
出处
《中国现代应用药学》
CAS
CSCD
1999年第6期41-43,共3页
Chinese Journal of Modern Applied Pharmacy
