摘要
为从分子水平上鉴定棉花品种的纯度和真实性,采用扩增带型清晰,多态性较高的SSR引物对51个常规棉品种进行了基因纯度鉴定、遗传聚类分析和品种特异性鉴定。基因纯度鉴定结果显示,69%的品种基因纯度较好,基因纯度在90%以上;12%的品种基因纯度较差,基因纯度在80%以下。聚类分析结果表明,所有品种分为2个大类和4个亚类,这反映出品种间的遗传基础较窄。品种特异性鉴定中,以遗传距离阈值T=0.01为划分标准,83%的品种具有特异性。这说明利用SSR标记可以有效鉴定棉花品种的基因纯度和特异性。
In order to identify cotton variety and test seed purity at the molecular level,gene purity and variety specificity of 51 cotton（G.hirsumtum L.） varieties were characterized by SSR primer pairs with high polymorphisms and stable amplified bands.The results showed that：most（69%） of the tested varieties with gene purity greater than 90%,while 12%of them with gene purity less than 80%.Genetic cluster analysis based on the marker genotypes indicated that all of the tested varieties were divided into two groups and four sub-groups.And 83%of the tested varieties were distinct one another using a genetic distance threshold of 0.01.All the results suggested that the SSR markers were suitable for and effective on characterization of gene purity and variety specificity of cotton varieties.
出处
《中国农学通报》
CSCD
北大核心
2011年第30期49-54,共6页
Chinese Agricultural Science Bulletin
基金
国家转基因生物新品种培育重大专项(2009ZX08005-018B)
农业部农产品质量安全监管(种子管理)项目"棉花品种分子检测技术研究"
关键词
棉花
SSR
基因纯度
聚类分析
特异性
cotton
SSR
gene purity
cluster analysis
variety specificity
