摘要
以改性分子筛作为固定化酶的载体，用固定化葡萄糖淀粉酶生产葡萄糖．一种新的基于多次吸附多次戊二醛交联的固定化方法增加了固定化葡萄糖淀粉酶的稳定性，另讨论了该方法的机理．制备的固定化葡萄糖淀粉酶在柱反应器中水解淀粉连续使用22d，产物DE值维持在95左右基本保持不变．
The modified molecular sieve were taken as support for the immobilization of glucoamylase (E. C. 3.2. 1. 3) to produce glucose. A new immobilization technique based on multiple times adsorbing and cross-linking was developed to improve the stability of the immobilized enzyme and the mechanism of immobilization was discussed. The immobilized enzyme prepared via this technique continuously hydrolyzes starch in a column reacter for 20 days keeping DE value about 95.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1999年第6期644-647,651,共5页
Journal of Fudan University：Natural Science
基金
国家自然科学基金!29673015
关键词
改性
分子筛
葡萄糖淀粉酶
固定化方法
制备
modified molecular sieve
glucoamylase
immobilization techniquel multiple times adsorbing and cross-linking
