摘要
以项目组已克隆并在GenBank中登记的棉花竹的FsMyb1基因(GU390881)序列为基础,利用生物信息学分析方法,对其编码的FsMyb1蛋白进行理化性质、分子结构、生物功能预测和分析。结果显示,棉花竹FsMyb1基因编码是一种亲水性蛋白质;无信号肽,无导肽,无跨膜结构域;亚细胞定位为细胞核;共有2个糖基化位点和21个磷酸化位点。二级结构包括无规则卷曲、α-螺旋、延伸链;三级结构的构象为α-helix/loop混合型构象,是典型的R2R3-Myb转录因子。同源比对和进化分析结果显示,FsMyb1蛋白与OsMyb等蛋白有很高的同源性。初步推测,FsMyb1蛋白可能是一类调节苯丙氨酸代谢,尤其是一种与调节次生细胞壁增厚和花青素生物合成功能相关的转录调节因子。
In current study, taking the sequences of FsMybl gene of Fargesia fungosa with the accession number GU390881 in GenBank as the study material, several parameters of protein coded by the gene including physico- chemical property, molecular structure and function, were analyzed and predicted by bioinformatics tools. The results showed that FsMybl gene encoded a kind of hydrophilic protein, without signal peptide, leader peptide and transmembrane topological structure. The subcellular localization was the nucleus. There were 2 glycosylation sites and 21 phosphorylate sites in the amino acid sequences. Secondary structure had random coil, alpha helix, extended strand. Tertiary structure was a mix conformation of alpha helix/loop, a typical R2R3 - Myb transcription factor. The results of phylogenetic analysis showed that FsMybl was homologous to OsMyb with quite high support. These results suggested FsMybl protein might be a kind of transcription factor which regulated phenylalanine metabolism, especially regulating secondary cell wall thickening and anthocyanins synthesis.
出处
《西部林业科学》
CAS
2011年第4期5-11,共7页
Journal of West China Forestry Science
基金
国家林业局948项目(2008-4-30)"竹子重要性状的基因工程研究技术引进"
云南省科技计划项目2009CD073
云南省中青年学术技术带头人后备人才培养项目(2010CI016)共同资助研究
