摘要
目的:研究不同产地、不同规格党参药材中党参炔苷、总多糖、总皂苷的含量,对其测定结果进行比较,分析三者之间的相关性,为评价和控制党参药材质量提供参考依据。方法:采用HPLC法测定党参炔苷,采用硫酸-苯酚比色法测定总多糖成分,采用香草醛-高氯酸比色法测定总皂苷。结果:党参炔苷在0.0085～0.425mg.mL-1(r=0.9998)范围内与峰面积呈良好线性关系,精密度良好,平均回收率100.7%;总多糖、总皂苷测定精密度及回收率亦符合要求。测定了5个不同产地、9种规格的党参药材样品。结论:党参样品中党参炔苷与总皂苷含量相关,与总多糖不成正比,这为科学评价和有效控制其质量提供了依据。
Objective ： To determine content of lobetyolin and total polysaccharides and total saponins from different region of Radix Codonopsis, and to explore the correlation between them so as to control the quality of Radix Codonopsis. Methods ： Diamonsil C18 column （ 4.6mm × 25 cm, 5 μm） were used, the mobile phase was acetonitrile - water （ 25 ： 75 ） and the UV detector was set at 268nm. Use Colormetric method was used to determine the total polysaccharides and total saponins content. Results ： The calibration curve was linear in the range of 0. 0085 - 0. 425mg. mL^-1 （ r = 0. 9998 ） , the average recovery ratio was 100.7%. The contents of lobetyolin in codonopsis from 5 habits were detected between 0. 187mg/g and 1. 314 mg/g. The precision and recovery ratio fits the requirement. Conclusions： There was certain correla- tion between lobetyolin and total saponins, but total polysaccharides not.
出处
《中华中医药学刊》
CAS
2011年第12期2779-2781,共3页
Chinese Archives of Traditional Chinese Medicine
关键词
党参
党参炔苷
相关性
Radix Codonopsis
Lobetyolin
HPLC
