摘要
为了挖掘谷子的抗旱基因,根据小麦转录因子基因TaDREB6的序列设计引物,从谷子中分离到一个DREB基因的cDNA序列,长1 113 bp,编码259个氨基酸,具有DREB类转录因子典型的N-末端碱性核定位信号和高度保守的AP2/ERFBP结构域。植物DREB类氨基酸序列多重比较分析表明,谷子DREB基因属于DREB2类转录因子,暂命名为SiDREB2。半定量RT-PCR表达模式分析表明,在谷子幼苗干旱胁迫过程中,谷子SiDREB2基因在正常生长情况下有低水平的表达,在干旱胁迫期间诱导上调表达,说明谷子SiDREB2基因的表达受干旱胁迫诱导,可能是谷子抗旱节水的关键基因。该基因的克隆为进一步探讨其应用于农作物抗旱节水性的遗传改良奠定了基础。
A DREB gene was amplified from Foxtail millet by PCR using a pair of primers designed on the sequences of Triticum aestivum TaDREB6 gene,the foxtail millet DREB gene was 1 113 bp in length,encoding 259 amino acids with basic amino acid regions possible nuclear location sequences（NLSs） and a highly conserved AP2/EREBP domain in the encoded putative protein.Multiple alignment analysis based on the amino acids encoded by DREB genes in plants showed that the Foxtail millet DREB gene was a member of DREB2 transcription factor family,and designed as SiDREB2.The expression patterns of SiDREB2 during drought stress were investigated by means of semi-quantitative RT-PCR.The results showed that the expression patterns of SiDREB2 during drought stress was up-regulated,which suggested that it was involved in the response of foxtail millet to drought stress and might be one of the key genes for drought tolerance and water use efficiency of foxtail millet.The cloning of this gene may provide the potential for its utilization in the improvement of drought tolerance and water use efficiency in other plants.
出处
《干旱地区农业研究》
CSCD
北大核心
2011年第5期69-74,共6页
Agricultural Research in the Arid Areas
基金
863重点项目子课题(2006AA100201
2006AA100223)
科技部973计划前期项目(2006CB708208)
高等学校学科创新引智计划项目(111-2-16)
关键词
谷子
DREB
干旱复水
表达模式
进化分析
foxtail millet
DERB transcription factor
drought and re-watering
express profile
phylogenetic analysis1
