摘要
目的:探讨机动车尾气有机提取物的体外遗传毒性作用。方法:用双核细胞微核试验和彗星试验评价机动车尾气有机提取物(2.5、5.0和10.0mg/L)对HepG2细胞的遗传毒性,另设溶剂对照组和阳性对照组,每组6个平行孔。结果:各组双核细胞微核率和Olive尾矩比较,差异有均统计学意义(F=18.608和9.525,P均<0.001)。5.0和10.0mg/L有机提取物组双核细胞微核率高于溶剂对照组,3个剂量组Olive尾矩均高于溶剂对照组(P均<0.05)。结论:机动车尾气有机提取物对HepG2细胞具有明显的遗传毒性。
Aim：To explore the genotoxicity of organic extract of vehicle exhaust on HepG2 cells in vitro.Methods：Cytokinesis-block micronucleus assay and comet assay were used to evaluate the genotoxicity of organic extract of vehicle exhaust（2.5,5.0 and 10.0 mg/L）on HepG2 cells.The solvent control group and positive control group were used as control.Results：There were significant differences in frequency of cytokinesis-block micronucleus and Olive tail moment among five groups（F=18.608 and 9.525,P0.001）.A significant increased frequency of cytokinesis-block micronucleus was observed in both 5.0 and 10.0 mg/L dose group compared with the solvent control group（P0.05）.A significant increased Olive tail moment was observed in three dose groups compared with the solvent control group（P0.05）.Conclusion：The organic extract of vehicle exhaust has a distinct genotoxicity on HepG2 cells in vitro.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2011年第6期904-906,共3页
Journal of Zhengzhou University(Medical Sciences)
关键词
机动车尾气
双核细胞微核
彗星试验
遗传毒性
vehicle exhaust
cytokinesis-block micronucleus
comet assay
genotoxicity
