摘要
目的应用微量离心柱法检测甲胎蛋白异质体(AFP-L3),分析AFP-L3在肝癌鉴别诊断中的意义。方法选择170例临床检测AFP为阳性的肝病患者血清,应用微量离心柱法分离洗脱获的AFP-L3,采用配套试剂罗氏E170电化学发光免疫分析仪检测分离前AFP和分离后AFP(AFP-L3),计算AFP-L3在AFP中的含量百分比。结果以AFP-L3≥10作为阳性判断标准,HCC患者血清中AFP-L3水平及阳性率明显高于肝硬化和慢性肝炎患者(P<0.01)。结论新微量离心柱法测定AFP-L3(%)对肝癌的诊断具有高特异性。
Objective To analyze significance of the differential diagnosis of hepatic carcinoma and detect Alpha-fetopreteins heterogeneity（AFP-L3） using microspincolumn coupled with lens culinaris agglutinin.Methods The Serum Samples were collected from 170 patients with AFP positive diagnosed with benign and malignant liner diseases.AFP-L3 was separated by using microspincolumn.The levels of AFP and AFP-L3 were determined by electro-chemiluminesent method and the ratio of AFP-L3 and AFP was calculated.Results AFP-L310% was taken as a diagnoatic indicator.The levels of AFP-L3 in 75 cases with hepectic carcinoma increase significantly compared with 95 cases of kenign liver diseases（P0.01）.Conclusion The detection of AFP-L3 using microspincolumn has the important significance of the differential diagnosis of hepatic carcinoma and benign liver diseases.
出处
《当代医学》
2011年第36期113-114,共2页
Contemporary Medicine
关键词
甲胎蛋白
甲胎蛋白异质体
肝癌
Alpha-fetoproteins
Alpha-fetoproteins heterogeneity
Hepatic carcinoma.
