摘要
目的:探讨皖南产蝮蛇毒(AHV)蛋白质C激活物(Protein C Activator,PCA)组分抗血栓效应。方法:家兔40只,随机分为4组,每组10只,颈总动脉取l ml注入血栓形成仪4个(硅化)聚乙烯管中,各管中分别含50、30、10μl(0.6216 mg/ml)蝮蛇毒PCA组分及50μl生理盐水,37℃条件下以16 r/min在恒流泵上旋转制备血栓;SD大鼠60只,随机分为假手术(SH)组、心肌梗死模型(MI)组(月桂酸钠左心室注入造模)、PCA干预组(MI+PCA组分为0.5 mg/kg、2 mg/kg、8 mg/kg 3个剂量组)、阳性对照组(阿司匹林5mg/kg),每组10只,各实验组动物均于术后3 h颈总动脉取血,按同样方法制备血栓;血液离心,分离血浆检测凝血功能。结果:蝮蛇毒PCA组分大、中、小3剂量组与对照组相比较,血栓的干、湿重量及长度均比对照组减轻(P<0.05);MI+PCA组(2 mg/kg、8 mg/kg)与MI组比较,血栓的干、湿重量长度均有明显差异(P<0.05),与阳性对照组无差异(P>0.05);MI+PCA组(2 mg/kg、8 mg/kg)与MI组比较,PT、APTT均明显增高(P<0.05),FIB显著降低(P<0.05),TT差异无统计学意义(P>0.05)。结论:AHV粗毒中分离纯化的PCA组分可明显干预血栓的形成,其可能通过PCA激活蛋白质C(Protein C)改变凝血功能。
Objective：To explore the antithrombotic effect of protein C activator（PCA） from the venoms of Agkistrodon halys found in south Anhui province.Methods：Forty rabbits were randomized into four groups（n=10 for each）,and 1 ml of blood was obtained from each group via carotid artery and respectively injected into four polyethylene tubes in which three contained 50,30,10 μl（0.6216 mg/ml） Agkistrodon snake venom PCA components by dose of large,medium and low and 50 μl normal saline for blood clots that were prepared in a constant flow pump by 16 r/min below 37 ℃.Sixty SD rats were randomly divided into groups of sham operation（SH）,myocardial infarct（MI） model,PCA intervention of three（MI＋PCA with divided dose of 0.5 mg/kg,2 mg/kg,and 8 mg/kg） and positive controls（aspirin at dose of 5 mg/kg）（n=10 for each）.The blood was obtained via carotid artery from the experimental animals three hours after intervention for blood clots as procedures described and determination of coagulation indexes.Results：Compared with the control group,the weight by dryness and wetness and length of the thrombi were found degraded in the three groups by snake venom PCA intervention（P0.05）.MI ＋ PCA group（2 mg/kg,8 mg/kg） were significantly different from the MI models regarding weight by dryness and wetness and dimension（P0.05）,which had no difference as compared with positive controls（P0.05）.Additionally,significantly elevated PT and APTT as well as reduced FIB were seen in MI＋PCA group（2 mg/kg,8 mg/kg） compared with MI models（P0.05）,yet TT remained no change（P0.05）.Conclusion：PCA components from the venoms of Agkistrodon halys can significantly produce antithrombotic effect through activated protein C by exerting an anticoagulant action.
出处
《皖南医学院学报》
CAS
2011年第6期440-443,共4页
Journal of Wannan Medical College
基金
安徽省高等学校省级自然科学研究项目(KJ2010B461)
院中青年科研基金项目(WK200918)
关键词
蝮蛇毒
PCA
抗血栓
实验研究
Agkistrodon halys venom
protein C activator
anti-thrombosis
experimental study
