摘要
建立了LC-MS/MS法测定贝母素甲在家兔血浆中的含量,研究灌胃及静注给予贝母素甲后家兔体内的药动学特征。以卡马西平为内标,采用C_(18)色谱柱,以乙腈-10 mmol/L甲酸铵溶液(35:65)为流动相,ESI源正离子模式,多反应离子(MRM)检测,监测离子对m/z 432.4→414.4(贝母素甲)和m/z 237.1→194.2(卡马西平)。贝母素甲在0.8～200 ng/ml浓度范围内线性关系良好,提取回收率88.5%～98.3%,日内、日间RSD均小于15%。家兔灌胃及静注给予贝母素甲后体内分别呈二室及三室开放模型,主要药动学参数如下:c_(max)(48.31±7.40)和(84.39±15.39)ng/ml,AUC_(0→∞)(270.08±80.17)和(50.72±14.02)ng·ml^(-1)·h^(-1),t_(1/2)(6.38±3.11)和(2.19±1.07)h。
A LC-MS/MS method was established for the determination of peimine in rabbit plasma. The pharmacokinetics ofpeimine in rabbits after ig and iv administration were investigated. A C,8 column was used, with the mobile phase of acetonitrile-10 mmol/L ammonium formate solution （35 ： 65） and using carbamazepine as the internal standard. ESI was applied and operated in positive iron multiple-reaction monitoring mode. The detected ions were m/z 432.4→414.4 （peimine） and m/z 237.1 → 194.2 （carbamazepine）. The calibration curve was linear in the concentration range of 0.8 - 200 ng/ml. The extraction recoveries were 88.5 % The plasma drug concentration-time profiles corresponded to a - 98.3 %, with the intra- and inter-day RSDs less than 15 %. two compartmental model for ig administration and a three compartmental model for iv administration. The main pharmacokinetic parameters were as follows： Cmax （48.31±7.40） and （84.39±15.39） ng/ml, AUCo-∞ （270.08：580.17） and （50.72±14.02）ng.ml ^-1.h ^-1, 4/2 （6.38±3.11） and （2.19±1.07） h.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2011年第12期914-916,共3页
Chinese Journal of Pharmaceuticals
基金
国家自然基金项目(81060346)
江西省自然基金项目(2008GZY0115)
