摘要
用去离子水浸提茶叶中的EGC,用高效液相色谱仪检测EGC并计算浸出率。在单因素实验基础上,利用Box-Benhnken实验设计进行三因素三水平的响应面分析实验,获得最优浸提条件:料液比1∶12、pH6.2、温度78℃、时间18 min、浸提2次。
A Method for extracting EGC from tea using deionized water was introduced. The contents of EGC were tested by HPLC. On the basis of single factor experiment, the response surface methodology with three factors at three levels was adopted by Box-Benhnken design. The optimized extraction conditions were: ratio of solvent to material = 1:12, pH = 6.2, extraction temperature = 78℃, extraction time = 18min and extracted for two times.
出处
《茶叶》
2011年第4期221-224,共4页
Journal of Tea
基金
杭州市农业科研攻关项目(20091832B05)
关键词
响应面法
EGC
浸提
Response surface methodology
EGC
Extraction