摘要
目的:建立一套发根农杆菌高频转化菘蓝植物的有效方法。方法:采用ATCC15834和RI1601两种发根农杆菌并通过超声波处理对草本植物菘蓝的下胚轴、子叶、带子叶下胚轴进行遗传转化,比较了共培养与否对毛状根诱导的影响,并进行了不同抗生素浓度的抑菌效果筛选和毛状根增殖培养基的筛选。结果:浸染后的外植体在不经过共培养阶段的情况下,诱导后的外植体成活率高,毛状根发根时间早,发根率高;经超声波处理后的外植体其毛状根诱导率比未处理高一倍多;同时,筛选出抗生素浓度为400 mg/L时为毛状根生长的最佳抑菌浓度;毛状根在液体培养基中的增殖速度是固体培养基的2～3倍,是普通根的37倍。结论:利用新的方法成功建立了菘蓝的毛状根诱导体系。
Objective： To build up a method of efficiently transforming Isatis indigotica with the Agrobacterium. Methods： Two kinds of Agrobocterium： ATCC 15834 and RI1601 were used to treat different fraction of Isatis indigot＆a. Explored the effect of cocultured, different antibiotic concentration and the media on multiply the hairy roots. Results：The explants with out coculture had had higher survival rate rooting rate and earlier sprout time. After ultrasonic treatment of plant, the indacement rate was two times than that of untreated one;The best antibiotic con centration was 400 mg/L;The proportion of the grow speed of the hairy root in the liquid culture media was 2 - 3 times than that of solid culture media, and 37 times of common roots. Conclusion ： The method is useiul for establishing an efficiently transformatiem system of Isatis indigotica by Agrobacterium.
出处
《中药材》
CAS
CSCD
北大核心
2011年第10期1486-1489,共4页
Journal of Chinese Medicinal Materials
基金
四川省科技支撑计划(2010SZ0007)
关键词
发根农杆菌
菘蓝
毛状根
Agrobacterium
lsatis indigotica Fort
Hairy root
