摘要
目的研究分解前、后白术挥发油对人类卵巢癌细胞SKOV-3细胞形态、生长抑制率、细胞凋亡及细胞周期的影响。方法使用倒置显微镜及采用四甲基偶氮唑蓝(MTT)法和流式细胞术,观察不同浓度分解前、后白术挥发油作用SKOV-3细胞不同时间后,对细胞形态、生长抑制率、细胞凋亡及细胞周期的影响。结果镜下可见凋亡小体。分解前、后白术挥发油各浓度样品作用细胞时间72h,或100,200mg/L作用细胞24,48h时,对细胞增殖均有显著影响(P<0.01)。浓度12.5,25,50mg/L分解后白术挥发油作用细胞24,48h时,对细胞增殖有显著影响(P<0.05)。浓度25,50mg/L分解前白术挥发油作用细胞24,48,72h时的细胞增殖抑制率明显高于分解后挥发油的抑制率,差异有统计学意义。随着分解前、后挥发油作用浓度的增加,分解前白术挥发油早期细胞凋亡率增高,晚期凋亡率减少;分解后白术挥发油早期细胞凋亡率、晚期细胞凋亡率增加;分解前挥发油G0/G1期和S期的细胞比例降低,G2/M期的细胞比例升高;分解后挥发油G0/G1期和G2/M期的细胞比例降低,S期的升高。结论分解前、后白术挥发油对SKOV-3细胞均具有杀伤抑制作用,其作用与时间和剂量呈正相关。在一定浓度范围,分解前白术挥发油对SKOV-3细胞增殖影响明显大于分解后。分解前、后白术挥发油均具有诱导细胞凋亡作用。分解前白术挥发油主要在凋亡早期发挥作用,分解后的挥发油在凋亡早期、晚期均发挥作用;分解前挥发油可将细胞阻滞在G2/M期,分解后可将细胞阻滞在S期。
Objective To investigate the effects of pre-and post-decomposed Rhizoma Atractylodis Macrocephalae volatile oil on the cell morphology, apoptosis induction and cell cycle in human epithelial ovarian cancer cell line SKOV-3. Methods Using fluorescence microscopy, MTT assay and flow cytometry, we investigated the effects of pre-and post-decomposed volatile oil at different levels on cell morphology, growth inhibition, cell apoptosis and cycling of cell line SKOV-3 with different treatment duration. Results Under fluorescence microscopy, we could clearly see the apoptosis bodies. They showed very significantly proliferation inhibition effects as using every pre-and post-decomposed volatile oil concentration samples to treat cell 72 h, or using 100 and 200 mg/L concentration samples to treat cell 24 h and 48 h respectively（P0.01）. There were significantly proliferation-inhibitory effects with 12.5, 25 and 50 mg/L post-decomposed volatile oil treatment on cells for 24 h and 48 h separately（P0.05）. There were significant difference between pre-decomposed volatile oil cell proliferation inhibition rates and that of post-decomposed volatile oil as using the 25 and 50 mg/L concentration pre-and post-decomposed volatile oil samples to treat cell in 24 h, 48 h and 72 h separately, the pre-decomposed volatile oil cell proliferation inhibition rates were obviously higher than that of post-decomposed volatile oil. Along with increasing concentration, the early phase apoptosis rates increased and late phase apoptosis rates decreased for pre-decomposed volatile oil; the early phase apoptosis rates increased and late phase apoptosis rates increased for post-decomposed volatile oil; the proportion of G0/G1 and S phases cell decreased and that of G2/M phases cell increased for pre-decomposed volatile oil, and the proportion of G0/G1 and G2/M phases cell reduced and that of S phases cell increased for post-decomposed volatile oil. Conclusion The pre-and post-decomposed volatile oil show proliferation-inhibitory effects on the cell line SKOV-3 in a time-and dose-dependent manner. Within a certain range of concentrations, the inhibitory effects of pre-decomposed volatile oil on cell proliferation were clearly higher than those of post-decomposed volatile oil. Both the pre-and post-decomposed volatile oils may induce apoptosis in SKOV-3 cells. The pre-decomposed volatile oil acts on early apoptosis phase and post-decomposed volatile oil shows these effects during the early and late apoptosis phases. The pre-decomposed volatile oil arrests cell cycle progression at G2/M phase, while post-decomposed volatile oil shows such effect at S phase.
出处
《中国药物与临床》
CAS
2011年第12期1372-1375,共4页
Chinese Remedies & Clinics
基金
山西省卫生厅科技攻关项目(200833)
关键词
白术
卵巢肿瘤
细胞凋亡
细胞周期
Atractylodes macrocephala
Ovarian neoplasms
Apoptosis
Cell cycle
