摘要
目的:比较地塞米松和他克莫司对HaCaT细胞热休克蛋白90(ISP90)表达的影响,并探讨其潜在的临床意义。方法:以含10^(-6)mol/L地塞米松或10^(-6)mol/L他克莫司的DMEM(高糖)培养基培养细胞,在24 h和48 h的不同时相点,采用Real timePCR和Western blotting检测tSP90的表达。结果:地塞米松作用24 h后,HSP90 mRNA和蛋白表达水平均升高,48 h后进一步升高(P<0.05);而他克莫司作用于HaCaT细胞后的不同时相点,tSP90 mRNA和蛋白水平与未处理组相比差异均无统计学意义(P>0.05)。结论:地塞米松可上调角质形成细胞中HSP90的表达,他克莫司不影响角质形成ll胞HSP90的表达。
Objective： To investigate the effects of dexamethasone or tacrolimus on heat shock protein 90 （HSP90） expression in HaCaT cells in vitro. Methods： The expression of HSP90 of the HaCaT cells treated with dexamethasone at 10^-6 mol/L or tacrolimus at 10^-6 mol/L were assessed by RT-real time PCR and Western blotting at settled duration. Results： Comparing with negative control, the expression of HSP90 was significantly increased in the cells after the 24 h and 48 h treatment of dexam- ethasone （P〈 0.05）, whereas the effect was not observed in the cells treat with tacrolimus. Conclusions： Dexamethasone could up-regulate the HSP90 expression in HaCaT cells, but tacrolimus did not.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2012年第1期11-13,共3页
Journal of Clinical Dermatology
基金
第三军医大学第二附属医院1520人才基金(2006-188号)资助项目
