摘要
目的:观察佐剂关节炎大鼠外周血血小板活化因子(platelet-activating factor,PAF)、白细胞介素6(interleukin-6,IL-6)、白细胞介素17(interleukin-17,IL-17)水平的变化及新风胶囊对其的影响。方法:将40只雄性Sprague-Dawley大鼠随机分为正常对照组、模型对照组以及甲氨喋呤(methotrexate,MTX)、雷公藤多苷片(Tripterygium Wilfordii polycoride Tablet,TPT)和新风胶囊治疗组,每组8只。除正常对照组外,大鼠右后足跖皮内注射弗氏完全佐剂0.1mL诱发大鼠关节炎。模型成功后,各组采用相应药物治疗30d。采用酶联免疫吸附法检测每组大鼠外周血PAF、IL-6和IL-17的含量,并观察各组大鼠体质量的变化,计算足跖肿胀度和关节炎指数。结果:与正常对照组相比,模型对照组大鼠外周血PAF、IL-6、IL-17含量,足跖肿胀度,关节炎指数显著升高(P<0.01)。与模型组相比,各治疗组大鼠PAF、IL-6、IL-17含量,足跖肿胀度,关节炎指数显著降低(P<0.01)。与MTX组、TPT组相比,新风胶囊组大鼠体质量显著升高,外周血PAF含量显著降低(P<0.05)。佐剂关节炎大鼠外周血PAF含量与外周血IL-6、IL-17含量,足跖肿胀度,关节炎指数呈正相关(P<0.01)。结论:佐剂关节炎大鼠外周血PAF含量升高,并与IL-6、IL-17水平,足跖肿胀度,关节炎指数具有相关性。新风胶囊通过下调PAF的水平,抑制血小板活化引起的炎症反应,降低IL-6、IL-17,从而降低佐剂关节炎大鼠的足跖肿胀度、关节炎指数。
Objective： To observe the levels of platelet-activating factor （PAF）, interleukin-6 （IL-6） and IL-17 in peripheral blood of rats with adjuvant arthritis （AA）, and the effects of Xinfeng Capsule （XFC）, a compound traditional Chinese herbal medicine.Methods： A total of 40 male Sprague-Dawley rats were randomized into normal control group, model group, methotrexate （MTX） group, Tripterygium Wilfordii polycoride Tablet （TPT） group, and XFC group, respectively. AA was induced in rats by intracutaneous injection of 0. 1 mL Freund＇s complete adjuvant in the right hindlimb. Contents of PAF, IL-6 and IL-17 in peripheral blood were measured by enzyme-linked immunosorbent assay. Body weight of rats, paw swelling and arthritis index （AI） were also observed. Results： Compared with the normal control group, the levels of PAF, IL-6 and IL-17 in peripheral blood, paw swelling and AI were significantly increased in the model group （P〈0. 01）. Compared with the model group, levels of PAF, IL-6 and IL-17, paw swelling and AI of the three drug-treated groups were significantly decreased （P〈0.01）. The body weight of the XFC group was significantly higher than those of the MTX- and TPT-treated groups. Compared with the MTX- and TPT-treated groups, level of PAF in the XFC group was significantly increased （P〈0. 05）. The level of PAF in peripheral blood of the AA rats was positively correlated with IL-6 and IL-17 levels in peripheral blood, paw swelling and AI （P〈0.01）.Conclusion： The expression of PAF increases in peripherai blood of rats with AA, and it correlates with IL-6, IL-I？, paw swelling and AI. XFC can decrease the expression of PAF, restrain infection induced by the activation of platelets, decrease the levels of IL-6 and IL-17, and hence decrease the paw swelling of rats with AA.
出处
《中西医结合学报》
CAS
2011年第12期1347-1352,共6页
Journal of Chinese Integrative Medicine
基金
国家中医药重点学科中医痹病学建设项目(No.国中医药发[2009]30号)
安徽省卫生厅中医药科研项目(No.2009ZY05)
