摘要
采用分光光度法在550 nm波长下以齐墩果酸为对照品测定黑灵芝中性提取物中总三萜含量,测得黑灵芝中性组分中三萜含量为40.92%,RSD为3.28%,平均加标回收率为103.9%。通过对DPPH自由基清除作用、总还原能力、对β-胡萝卜素-亚油酸的抑制作用和对亚铁离子螯合能力的测定,评估了黑灵芝中性组分的抗氧化性。结果表明:它具有较强的抗氧化性。另外,用Folin-Ciocalteu酚方法测得黑灵芝中性组分的总酚含量为31 mg/g中性组分。据此,为黑灵芝质量评价及其中性组分开发应用提供一定的理论指导。
Triterpenoids content of neutral components extracted from Ganoderma atrum was determined by spectrophotometry at 550 nm. The triterpenoids content of the neutral components was 40.92% with RSD = 3.32%, and its recovery was 103.9%. The antioxidant capacity of the extract was assessed by scavenging effects on 2,2-di- phenyl-l-picrylhydrazyl radicals （DPPH）, reducing power assay, β-carotene bleaching assay and metal chelating activity on ferrous ions. The results indicated that the neutral components isolated from Ganoderma atrum had signif- icant antioxidant activity. Total phenols content determined by Folin-Ciocaheu colorimetric method was 0.31 mg/g neutral components. It will provide certain theoretical guidance for the quality evaluation and application of Ganoder- ma atrum.
出处
《南昌大学学报（工科版）》
CAS
2011年第4期332-337,共6页
Journal of Nanchang University(Engineering & Technology)
基金
国家"863"计划项目(2008AA10Z325)
食品科学与技术国家重点实验室目标导向资助项目(SKLF-MB-201001)
关键词
黑灵芝
中性组分
三萜
分光光度法
抗氧化性
Ganoderma atrum
neutral components
triterpenoids
spectrophotometry
antioxidant activity
