摘要
用荧光光谱法、紫外-可见分光光度法在生理条件下(pH=7.41)研究了β-环糊精(β-CD)、6-去氧-(N-胺乙基)环糊精(CR-β-CDen)分别与刚果红(CR)的包合作用;研究了CR,(CR-β-CD)和(CR-β-CDen)超分子体系与小牛胸腺DNA的相互作用。紫外滴定实验结果表明:(1)β-环糊精和6-去氧-(N-胺乙基)环糊精都与客体分子刚果红发生超分子作用;(2)CR,CR-β-CD,CR-β-CDen与小牛胸腺DNA作用的结合常数Kb值分别为4.1×104L/mol,1.36×105L/mol,2.33×105L/mol,表明超分子体系CR-β-CDen与DNA的相互作用最强。荧光实验结果表明三种化合物CR,CR-β-CD,CR-β-CDen的荧光强度随着CT-DNA的增加而增大,其中环糊精衍生物CR-β-CDen荧光增强效果是最好的。
Inclusion interaction of β-cyclodextrin （β-CD） and 6-mono- （N-aminoethyl） amino-β-cyclodextrin （9-CDen） with congo red and interaction of congo red （CR） and inclusion complexes β-CD/CR and β-CDen/CR with calf thymus DNA are researched by UV-vis and fluorescence spectra. The results show that the values of the binding constants （Kb） of CR/ DNA, CR-β-CD/DNA and CR-β-CDen/DNA are 4.1 × 10^4 L/tool, 1.36 × 10^5 L/mol and 2.33× 10^5 L/mol respectivly, which indicates that the interaction between CR-β-CDen and DNA is the strongest. The three complexes luminescence were enhanced with addition of CT-DNA. And the effect of fluorescence enhancement of CR-β-CDen is the best.
出处
《衡阳师范学院学报》
2011年第6期74-78,共5页
Journal of Hengyang Normal University
基金
Supported by the National Natural Science Foundation of China(21102040)
The Scientific Research Fund of Hunan Provincial Education Department(09K099)
The Foundation of science and technology of Hengyang(2010kj26)
Science Foundation of Hengyang Normal University of China(10B66)
Foundation for University Key Teacher by Hunan Provinc
