摘要
用NaCl浓度为0(对照),10,50,100,200,300mmol/L分别处理金盏菊,对不同盐浓度下金盏菊生长、抗氧化能力和盐胁迫蛋白的变化进行了研究。结果显示,在不同浓度NaCl胁迫下,与对照相比,金盏菊鲜重、干重、叶绿素含量、SOD和POD活性均呈先上升后下降趋势,而MDA含量呈先下降后上升趋势;Na+含量、细胞质膜透性呈上升趋势,而根系脱氢酶活性、K+含量、CAT活性则呈下降趋势。电泳结果显示,POD有8个谱带;10mmol/LNaCl处理诱导盐胁迫蛋白产生,而其他浓度盐胁迫均无盐胁迫蛋白诱导产生。因此,10mmol/L NaCl处理对金盏菊生长有一定促进作用,而随着NaCl浓度逐渐增加,其生长受抑制程度也逐渐加重。综合分析表明,金盏菊耐盐阈值为100mmol/L。
Calendula officinalis plants were treated with NaCl at different concentrations（0,10,50,100,200 and 300 mmol/L）,while fresh and dry weights,chlorophyll content,root dehydrogenase activity,Na＋ and K＋ content,activity of superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT）,MDA content,and cell membrane permeability were determined.Polyacrylamide gel electrophoresis（PAGE） of POD isoenzyme and SDS-PAGE of salt stress protein were done.Compared with the control,fresh and dry weight,chrolophyll content,SOD and POD activity initially increased but then decreased,while MDA content did the opposite.Na＋ content and cell membrane permeability increased,while root dehydrogenase activity,K＋ content and CAT activity decreased.Eight bands were detected through PAGE of POD isoenzyme.Only salt stress protein was induced under 10 mmol/L NaCl stress.Therefore,10 mmol/L NaCl treatment promoted the growth of C.officinalis but the inhibitory effects on C.officinalis growth were enhanced as NaCl concentrations increased.The synthesis analysis showed that the salt tolerance threshold of Calendula officinalis was 100 mmol/L.
出处
《草业学报》
CSCD
北大核心
2011年第6期52-59,共8页
Acta Prataculturae Sinica
基金
安徽省科技厅2009年度攻关计划项目(09020303081)
蚌埠市花卉科技专家大院(蚌科200968)
安徽科技学院重点学科(AKXK20101-3)资助
关键词
金盏菊
NACL胁迫
生长
抗氧化酶
膜稳定性
盐胁迫蛋白
Calendula officinalis
NaCl stress
growth
antioxidant enzyme
membrane stability
salt stress protein
