摘要
研究了不同超高压条件(0、50、150、300、450和600 MPa,保压处理15 min)对鳙感官、色度、硬度、pH、氨基氮含量、Ca2+-ATPase活性和超微结构的影响。结果表明,超高压处理改善了鱼肉的气味、滋味和咀嚼度,300、450和600 MPa处理能够显著提高鱼肉的感官品质(P<0.05);改变了鱼肉的色度,使L*值和b*值升高,a*值下降。150 MPa以上的高压能够显著提高鱼肉的硬度(P<0.05)。氨基氮含量在高压处理后显著升高(P<0.05),50和300 MPa处理组的氨基氮含量比对照组分别上升了15.19%和17.89%。鱼肉的pH随着压力的升高而上升。超高压抑制了肌球蛋白Ca2+-ATPase活性,当压力≥150 MPa时,Ca2+-ATPase的失活速度明显加快。超高压对肌原纤维的超微结构也有较大影响,150 MPa处理后,肌节收缩,肌原纤维变粗,M线、Z线和H区消失,A带和I带断裂。450 MPa处理后,肌节结构被完全破坏,肌原纤维间隙消失,肌原纤维凝胶化现象严重。综合感官和理化测定结果可知,超高压在改善鳙品质方面具有潜在的应用前景。
The effects of high pressure processing（HPP）（0,50,150,300,450 and 600 MPa for 15 min）on the sensory quality,color,firmness,pH,amino nitrogen,Ca2＋-ATPase activity and ultrastructure of Aristichthys nobilis muscle were investigated.The odor,taste and chewiness were modified by HPP,and the sensory quality improved significantly after treatment at 300,450 or 600 MPa（P〈0.05）.L＊ and b＊ value increased while a＊ reduced after HPP.The firmness of the muscle increased pronouncedly（P〈0.05）with pressure beyond 150 MPa.HPP treated samples had higher content of amino nitrogen（P〈0.05）compared to the control with increases of 15.19% and 17.89% for 50 and 300 MPa pressurized samples,respectively.pH showed a positive correlation with the pressure.The activity of Ca2＋-ATPase was inhibited by HPP,and pressures beyond 150 MPa inactivated Ca2＋-ATPase more rapidly.Moreover,HPP had a great impact on ultrastructure of A.nobilis muscle.After treatment at 150 MPa,sarcomeres contracted and myofibrils looked thicker than the control.In addition,disappearance of Z-line,M-line and H-zone and breakage of A-band and I-band were observed.The structure of sarcomeres was completely destroyed,intermyofibrillar space disappeared and gelation of myofibrils was observed when pressurized by 450 MPa.Our results indicated that the HPP could serve as a promising technique for commercial quality enhancement of A.nobilis muscle.
出处
《水产学报》
CAS
CSCD
北大核心
2011年第12期1897-1903,共7页
Journal of Fisheries of China
基金
嘉兴市科技局计划项目(2009BZ6004)
关键词
鳙
超高压
品质
Ca2+-ATPase
Aristichthys nobilis
high pressure processing（HPP）
quality
Ca2＋-ATPase
