摘要
目的检测急性分泌性中耳炎(acute secretory otitis media,SOM)患者外周血及中耳积液中IL-2、IL-4、IL-6、IL-10、TNF、IFN-γ的表达水平,以期综合分析免疫因素在其发病中的作用。方法采用BD CBA Flex SetIL-2、IL-4、IL-6、IL-10、TNF、γ-干扰素试剂盒,利用流式细胞术微球阵列法检测37例急性上呼吸道感染诱发急性分泌性中耳炎外周血、耳积液,8例鼻咽癌患者放疗后分泌性中耳炎耳积液和10例正常人血清样本中6种细胞因子水平。结果急性上呼吸道感染诱发的中耳积液中的IL-2、IL-4、IL-6、IL-10、TNF、IFN-γ表达水平显著高于外周血(P<0.05);其中IL-6、IL-10、TNF、IFN-γ水平明显高于放疗后耳积液水平(P<0.05)。急性分泌性中耳炎外周血上述六种因子较正常对照组外周血差异无统计学意义(P>0.05)。结论中耳粘膜参与了急性分泌性中耳炎患者局部的免疫反应,在其发病中可能起到一定作用。
Objective To detect the level of IL-2, IL-4, IL-6, IL-10, TNF, IFN-γin the peripheral blood and middle ear effusion in patients with secretory otitis media and determine the role of immune status in the pathogenesis of the disease. Methods The levels of the six cytokines in the serum and ear effusion from 37 cases of acute upper respirato-ry tract infection, 8 cases of nasopharyngeal carcinoma after radiotherapy with secretory otitis media and 10 cases of nor- mal persons were detected by using flow cytometry and BD CBA Flex Set kit. Results The level of IL-2, IL-4, IL-6, IL-10, TNF, IFN-γin acute upper respiratory infection-induced middle ear effusion was significantly higher than in pe-ripheral blood （P 〈 0.05）. The level of IL-6, IL-10, TNF, IFN-γ was significantly higher than in radiotherapy induced ear effusion （P 〈 0.05）. The level of six cytokines in peripheral blood of SOM patients showed no significant difference from the control group（P 〉 0.05）. Conclusion Middle ear mucosa is involved in local immune response and plays an important role in the pathogenesis of acute otitis media with effusion.
出处
《中华耳科学杂志》
CSCD
2011年第4期448-450,共3页
Chinese Journal of Otology
基金
广东省医学科研基金资助(No:A2009654)
