摘要
目的探讨鸡血藤黄酮类有效部位(spatholobus suberectus column extract,SSCE)对人非小细胞肺癌A549细胞凋亡及线粒体膜电位的影响。方法采用细胞增生试剂盒cell counting kit-8,观察SSCE对A549细胞增生的影响;通过Annexin V-PI双染法结合Hoechst33324染色检测SSCE对A549细胞凋亡的影响;应用Mitotracker Red及JC-1染色,检测SSCE对细胞线粒体及线粒体膜电位的影响。结果浓度高于40 mg/L的SSCE对A549细胞生长有抑制作用,且具有浓度时间依赖性。SSCE作用24 h后,A549细胞呈现典型的凋亡现象:膜磷脂酰丝氨酸外翻;细胞核呈浓染致密的固缩形态或颗粒状荧光;线粒体由棒状变成颗粒状,并向细胞核聚集;线粒体膜电位下降。结论 SSCE可以诱导人非小细胞肺癌A549细胞发生凋亡,改变线粒体形态且降低其线粒体膜电位。
Objective To investigate the effect of spatholobus suberectus column extract（SSCE） on apoptosis and mitochondrial transmembrane potential of non-small cell lung cancer A549 in vitro. Methods Cell counting kit-8 was used to investigate the effect of SSCE on the proliferation of A549,apoptotic morphological changes were observed by Annexin V-PI double-staining technique,nuclear changes were observed by Hochest33342 staining,mitochondrial changes were observed by Mitotracker red staining,and mitochondrial membrane potential was examined by JC-1 staining. Results The concentration of 40 mg/L,SSCE could suppress the proliferation of A549 cells in a dose and time-dependent manner.After cultured with SSCE for 24 h,A549 cells exhibited typical characteristics of apoptosis,such as phosphatidylserine（PS） externalization,pyknosis,dense-stained nucleus and cytoplasm,nucleus granulation,mitochondrias gathered to the nucleus and their shape changed from rod into granular form,mitochondrial membrane potential decreased. Conclusion SSCE induced apoptosis of A549 cells by changing the mitochondrial morphology and reducing the mitochondrial membrane potential.
出处
《首都医科大学学报》
CAS
北大核心
2011年第6期811-815,共5页
Journal of Capital Medical University
基金
北京市教育委员会科技发展计划(KM200810025008)~~
关键词
鸡血藤
A549
细胞凋亡
线粒体
spatholobus suberectus
A549
apoptosis
mitochondria
