摘要
在草鱼(Ctenopharyngodon idellus)饲料中分别添加0(Ⅰ组)、1×108(Ⅱ组)、3×108(Ⅲ组)、5×108cfu/g(Ⅳ组)芽孢杆菌(Bacillus spp.)持续投喂草鱼,收集粪便测定草鱼对各营养物质的表观消化率和消化酶活性。结果表明:投喂30d后,Ⅱ、Ⅲ组干物质、粗蛋白、粗脂肪表观消化率和Ⅳ组粗蛋白、粗脂肪表观消化率均显著高于Ⅰ组(对照组),其中Ⅱ组的表观消化率最高,且芽孢杆菌添加量与营养物质表观消化率最符合一元二次曲线方程。Ⅱ组中肠和Ⅱ、Ⅲ组肝胰脏蛋白酶活性均显著提高;Ⅱ组的前肠和后肠淀粉酶活性显著高于对照组;Ⅱ、Ⅲ组后肠脂肪酶活性均显著高于对照组。投喂60d后,消化酶活性也有相似的结果。双因子方差分析表明:芽孢杆菌的添加量对中肠、后肠和肝胰脏蛋白酶活性,前肠、中肠和后肠淀粉酶活性,中肠和后肠脂肪酶活性均有显著影响;投喂时间对肝胰脏蛋白酶、淀粉酶活性有显著影响。
Ctenopharyngodon idellus were fed with basal diet supplemented with 0（Ⅰ）、1×10^8（Ⅱ）、3×10^8（Ⅲ）、5×10^8cfu/g（Ⅳ） Bacillus spp. respectively for 30 days. Feces was collected to detect apparent digestibility of feed nutrients. The apparent digestibility of dry matter, crude protein and crude lipid in I group was significantly lower than the other treatment groups （P〈0. 05）. Quadratic curve e- quation was the best to fit the relationship between dosages of Bacillus spp. and apparent digestibility of nutrients. Protease activity of the mid-gut in Ⅱ group and the hepatopancreas in Ⅱ, Ⅲgroups increased significantly compared with I group （P〈0. 05）. Amylase activity of the fore-gut and hind-gut in Ⅱgroup increased significantly compared with I group （P〈0. 05）. Lipase activity of the hind-gut in Ⅱ, Ⅲ group increased significantly compared with I group （P〈0. 05）. After feeding for 60 days,the similar results appeared. Two-way analysis of variance showed that protease activity of the mid-gut, hind-gut and the hepatopancreas, amylase activity of the fore-gut, mid-gut and hind-gut, lipase activity of the mid- gut and hind-gut were all significantly affected by the additional doseages of Bacillus spp. （P〈0. 05）. Protease activity and amylase activity of the hepatopancreas were significantly affected by feeding days （P〈0. 05）.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2012年第1期106-111,共6页
Journal of Huazhong Agricultural University
基金
国家科技支撑计划项目(2007BAD37B02)
湖北省"十一五"科技攻关项目(2006AA203A02
2007AA203A01)
关键词
草鱼
芽孢杆菌
表观消化率
蛋白酶活性
淀粉酶活性
脂肪酶活性
Ctenopharyngodon idellus
Bacillus spp.
apparent digestibility
protease activity
amylase activity~ lipase activity
