摘要
为在兼顾生物量的基础上提高液体发酵中三萜的百分含量,首先通过单因子试验筛选出最佳的碳源、氮源、无机盐,然后采用均匀设计(UD)对其培养基配比和培养条件进行优化试验,建立适当的数学模型,并对模型进行验证确定优化条件.单因子试验结果表明玉米淀粉既能促进菌丝体的生长,又能促进三萜的合成;麸皮虽然对菌体生长略有不利,但是对三萜的积累作用特别显著;硫酸镁对菌丝体生长效果不显著,但是对三萜的合成比较有利.均匀设计试验结果表明,菌丝体干重最大的培养条件和三萜的最优培养条件相差甚远.菌丝体干重的最优培养条件为:1 L培养液中玉米淀粉47 g,麸皮47 g,硫酸镁0.5 g,初始pH 3.0,培养7 d;三萜最佳培养基配方为:1 L培养液中玉米淀粉20 g,麸皮20 g,硫酸镁1.85 g,初始pH 3.0,培养16 d.菌丝体干重在最优条件下能达到15.58(±0.37)g L-1;三萜百分含量在最优培养条件下高达6.04(±0.03)%,三萜的百分含量比基础发酵结果和报道的最高发酵结果3.18%提高了90%,因此。
Antrodia cinnamomea contains many valuable compounds, especially triterpenoids. The submerged culture conditions of Antrodia cinnamomea producing triterpenoids were optimized by one-factor-at-a-time and uniform design （UD） methods taking biomass into account. First, the one-factor-at-a-time method was adopted to investigate the effects of initial pH and variables of medium components （i.e., carbon, nitrogen and mineral sources） on triterpenoid production and mycelia growth in detail. Corn starch not only improved mycelia growth but also enhanced triterpenoid production, and was identified to be the most suitable carbon. Wheat bran was the favorable nitrogen sources to triterpenoid production （5.5%）, however, it was not good for biomass. MgSO4 was contributed to triterpenoid production, without significant effects on mycelia growth. Subsequently, the concentrations of corn starch, wheat bran, MgSO4, initial pH and culture time were optimized using the UD method. The optimal conditions for mycelia yield were determined as 4.7% corn starch, 4.7% wheat bran, 0.05% MgSO4, pH 3.0 and 7 days of cultivation, and the optimal conditions for triterpenoid production was 2% corn starch, 2% wheat bran, 0.185% MgSO4, pH 3.0 and 16 days of cultivation, being different from the former. Subsequent experiment confirmed the validity of the models. This optimized strategy in shake-flask culture led to a mycelia yield （Dry cell weight, DCW） of 15.58（±0.37） g L^-1 and triterpenoid percentage （POT） of 6.04（±0.03）%, respectively. The percentage of triterpenoids was considerably 90% higher than that obtained in the preliminary studies （3.18%）. The statistical methods used in the present study are useful strategies for optimizing culture conditions of liquid fermentation. Fig 5, Tab 3, Ref 34
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2011年第6期901-906,共6页
Chinese Journal of Applied and Environmental Biology
基金
深圳市中科海外科技有限公司研发与投资专项基金项目资助~~
关键词
樟芝
粗三萜
生物量
均匀设计
液体发酵
培养条件优化
Antrodia cinnamomea, crude triterpenoids, biomass, uniform design （UD）, liquid fermentation, culture condition, optimization
