摘要
利用RAPD分子标记技术对90份辣椒种质资源遗传多样性进行分析,从200个RAPD引物中筛选出10个引物分别对供试材料的DNA进行扩增,共扩增出70个位点,其中多态性谱带38条,多态性程度为54.3%。平均每个引物产生7条多态性谱带,每个引物可扩增出4～9条,谱带大小为100～2 000bp。对RAPD结果进行聚类分析,在D=0.33时,可以将供试的90份辣椒种质聚类为五大类群:第一类有85个种质;第二类有1个种质,是福建沙县的中牛角;第三类仅福建永安的永安黄椒1个种质;第四类有2个种质,分别是亚蔬的小尖椒PBC1752和PBC81;第五类有1个品种,是亚蔬的小线椒PBC932-6-2。品种间的亲缘关系较近,遗传基础较为狭窄。
Genetic polymorphisms of 60 Capsicum annuum L.cultivars were analyzed by using RAPD.Ten from 200 primers were selected for amplification.Seventy DNA fragments and 38 bands showed polymorphisms that occupied 54.3% of the entire amplified bands.Each primer could amplify 4 to 9,averaging 7 bands,polymorphic bands.Most of the amplified fragments had a length ranging from 100 to 2 000 bp.The cluster analysis using DPS software showed that the tested materials could be categorized into 5 groups at D=0.33.The first group included 85 varieties;the second group had only one species,i.e.,MLG horn from Shaxian,Fujian;the third group had also only the yellow pepper from Yongan,Fujian;the fourth group contained two cultivars,the small greens hot pepper PBC1752 and PBC81;and the last group,Xiaoxian pepper,PBC932-6-2.All of these speices were genetically closely related with little differentiations among them.
出处
《福建农业学报》
CAS
2011年第5期747-752,共6页
Fujian Journal of Agricultural Sciences
基金
福建省农业科学院青年人才基金(2009QC-11)
福建省财政专项-福建省农业科学院科技创新团队建设基金(STIFY06)
福建省科技计划重大专项(2008NY002)
关键词
辣椒
RAPD
遗传多样性
聚类分析
Capsicum annuum L.
RAPD
genetic diversity
germplasms
