摘要
以台州本地荸荠‘店头三王’球茎顶芽为外植体,以MS为基本培养基,添加不同浓度6-BA,NAA,AC,PVP等诱导剂,结果显示:适宜的诱导培养基为MS+BA 1.5 mg.L-1;适宜的增殖培养基为MS+6-BA1.5 mg.L-1+NAA 0.01 mg.L-1,增殖系数为11.2,适宜的生根培养基为1/2MS+NAA 0.05 mg.L-1+AC 1000 mg.L-1或者MS+NAA 0.05 mg.L-1+AC 1 000 mg.L-1,生根率达100%。并且提出了荸荠继代培养中以添加400 mg.L-1PVP防治褐化现象效果较好,株褐化率从89.6%下降到2.0%,增殖系数从11.20提高到13.85。采用本项目荸荠组培苗的驯化、移栽技术,假植秧田移栽成活率高达100%。
In order to establish a rapid propagation system of Eleocharis dulcis cv.Diantou Sanwang by tissue culture,the corm terminal buds of Diantou Sanwang was used as explants,and basic MS medium supplemented with 6-BA,NAA,AC and PVP at various concentrations was used.The results showed that the optimal induction medium was MS＋6-BA 1.5 mg·L-1.For proliferation of the shoots,MS＋6-BA 1.5 mg·L-1＋NAA 0.01 mg·L-1 was the best medium,and the highest proliferation rate reached 11.2.The optimal combination of rooting medium was modified as 1/2 MS＋NAA 0.05 mg·L-1＋AC 1 000 mg·L-1 or MS＋NAA 0.05 mg·L-1＋AC 1 000 mg·L-1 on which 100% rooting rate was induced.We also found that it could be better in controlling browning when we added 400 mg·L-1 PVP during subculture,and browning rate decreased from 89.6% to 2.0%,meanwhile,the multiplication coefficient increased from 11.20 to 13.85.In this paper,we also made a detailed study on the tissue culture acclimation and transplanting techniques,and the seedling survival rate was up to 100%.
出处
《浙江农业学报》
CSCD
北大核心
2011年第6期1123-1128,共6页
Acta Agriculturae Zhejiangensis
基金
台州市科技计划重点项目(071TG01)
关键词
荸荠
'店头三王’
组培
褐化
驯化
移栽技术
Eleocharis dulcis
Diantou Sanwang
tissue culture
browning
acclimation
transplanting technique
