摘要
目的:研究咳喘宁对病毒诱发支气管哮喘大鼠信号转导子和转录激活子6(STAT6)及其mRNA表达的影响。方法:幼年雄性SD大鼠60只随机分为磷酸盐缓冲液(PBS)对照组20只、模型组20只、地塞米松与沙丁胺醇治疗组(简称"地沙治疗组")10只和咳喘宁治疗组10只。建立卵白蛋白哮喘大鼠模型并以呼吸道合胞病毒激发哮喘。采用免疫组化法和原位杂交法分别检测STAT6蛋白和STAT6 mRNA的表达。结果:免疫组化和原位杂交法显示,模型组支气管STAT6蛋白和STAT6 mRNA的表达均高于磷酸盐缓冲液(PBS)对照组、地沙治疗组和咳喘宁治疗组,其主要表达细胞是上皮细胞;咳喘宁治疗组支气管STAT6蛋白和STAT6 mRNA的表达低于模型组(P<0.01),和地沙治疗组比较,咳喘宁治疗组支气管STAT6蛋白和STAT6 mRNA的表达略高,但两组差异无统计学意义(P>0.05)。结论:哮喘大鼠支气管STAT6及其mRNA较强表达,上皮细胞是其主要表达细胞;咳喘宁可下调哮喘大鼠支气管STAT6及其mRNA表达,可能为其防治病毒诱发哮喘的重要机制。
Objective： To study Kechuanning rat virus-induced bronchial asthma, signal transducer and activator of tran- scription 6（STAT6） and its mRNA expression. Methods： 60 young male SD rats were randomly divided into phosphate buffered saline （PBS） control group, 20, 20 model group, dexamethasone and salbutamol treatment group （referred to as ＂sand in the treatment group＂）, 10 and cough ning the treatment group 10. Established rat model of ovalbumin asthma and respiratory syncytial virus to stimulate asthma. Using immunohistochemistry and in situ hybridization were used to detect STAT6 protein and STAT6 mRNA expression. Results： Immunohistochemistry and in situ hybridization showed that the model group bronchial STAT6 protein and STAT6 mRNA expression were higher than the phosphate buffered saline （PBS） control group, to sand the treatment group and Kechuanning treatment group, the main expression of cell epithelial cells; Kechuanning treated bronchial STAT6 protein and STAT6 mRNA expression than the model group（P〈0.01）, and to sand the treatment group, treatment group, bronchial Kechuanning STAT6 protein and STAT6 mRNA expression was slightly higher, but the difference was not statistically significant （/%0.05）. Conclusion： The rat bronchial asthma STAT6 strong expression of its mRNA, the expression of epithelial cells are the main cells; cough would rather cut rat bronchial asthma and STAT6 mRNA expression, its prevention may be an important mechanism for virus-induced asthma.
出处
《中医药导报》
2011年第12期8-11,15,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(30901921)
关键词
哮喘
信号转导子
转录激活子6
咳喘宁口服液
Asthma
signal transducer
Activator of transcription 6
Kechuanning oral
