摘要
目的探讨祛风除湿中药独活对β淀粉样蛋白(amyloidβ protein,Aβ)脑内注射所致大鼠痴呆模型的保护作用。方法 24只雄性Wistar大鼠行颅内Aβ海马CA1区内注射术后,随机分为4组,独活组、吲哚美辛组、假手术组和模型组,每组6只,分别给予中药独活、吲哚美辛及生理盐水灌胃。进行水迷宫实验检测逃避潜伏期,在实验4周末处死大鼠,检测凋亡蛋白Bcl-2/Bax在大鼠脑中表达的情况。结果除假手术组外,其他3组逃避潜伏期均有不同程度延长(P<0.05);与模型组比较,其他3组Bcl-2/Bax大鼠脑中的表达有所不同,独活组可提高Bcl-2/Bax的比值(P<0.05)。结论独活可以改善痴呆模型大鼠的学习记忆能力,并能上调Bcl-2表达,抑制Bax表达,提高Bcl-2/Bax的比值,对痴呆大鼠神经细胞的凋亡有抑制作用。
Objective To investigate the protective effect of traditional Chinese medicine of expel wind-evil and remove wetness on the AD rat model established by a injection of Aβ into the brain.Methods After the injection of Aβ into the hippocampus--CA1 area of rats,twenty-four male Wistar rats were randomly divided into duhuo Group（DH group,n=6）,Western medicine Group（XYgroup,n=6）,Sham operation Group（JS group,n=6）and Model Group（MXgroup,n=6）.Then rats in each group were respectively given intragastric infusion with duhuo fluid,indometacin fluid and Sodium Chloride.At last,the rats were killed at the end of 4th week in order to detect the expression of Bcl-2、Bax in their brains.Results 1.The results of each group showed different fig.changes in the water maze experiment except Sham operation Group.2.The expressions of Bcl-2、Bax in the rats＇ brain of 4 groups were diverse.The group with duhuo fluid was able to increase the expression of Bcl-2/Bax in the brain（P0.05）.Conclusions Duhuo can improve the learning and memory abilities of AD model rats,and can also inhibit the apoptosis of nerve cells.
出处
《中华保健医学杂志》
2011年第6期459-460,共2页
Chinese Journal of Health Care and Medicine
基金
辽宁省教育厅课题(L2010372)
