摘要
采用自溶法、自溶 +酶解法、诱导剂 +自溶 +酶解法对S .CerevisiaeYJ酵母细胞进行生物处理使其释放蛋白质及其分解产物 .对反应产物α 氨基氮和细胞蛋白质溶出率及细胞干基提取率的测定结果表明 :诱导剂 +自溶 +酶解法的作用效果明显优于自溶 +酶解法及自溶法 .诱导剂+自溶 +酶解法比自溶 +酶解法及自溶法的反应产物中游离α 氨基氮分别提高了 9.0mg/g及2 3 .0mg/g(以干酵母计 ) ,细胞蛋白质溶出率分别提高了 1 3 .50 %及 2 9.83 % ,细胞干基提取率分别提高了 7.0 %及 2 0 .90 % .
In studies of a 5 L isothermal yeast hydrolytic tank, at 200 g/L cells,pH 6.5 and 50 ℃,S.cerevisiae YJ cells were treated biologically by autolysis or by autolysis plus enzymatic hydrolysis or by inducer plus autolysis plus enzymatic hydrolysis in order to release yeast cell protein and its decomposition product.The effect of inducer plus autolysis plus enzymatic hydrolysis was investigated with the result that the rate of lysis was obviously superior to autolysis plus enzymatic hydrolysis and autolysis through the determination of the α aminonitrogen,the dissolved rate of cell protein and the extraction rate of cell (dry basis )of the product obtained by the three methods. There were 9.0 mg/g (dry yeast) and 23.0 mg/g (dry yeast) increase of the free α amino nitrogen ,13.5% and 29.83% increase of the dissolved rate of cell protein, and 7.0% and 20.90% increase of the extraction rate of cell (dry basis )by inducer plus autolysis plus enzymatic hydrolysis as compared with by autolysis plus enzymatic hydrolysis and autolysis.
基金
浙江省科委重点科研项目! ( 951 0 89A)
关键词
自溶
水解
蛋白质溶出率
啤酒
啤酒酵母细胞
brewer’s yeast
autolysis
hydrolysis
α amino acid nitrogen
dissolved rate of protein
extraction rate of cell (dry basis)
