摘要
研究陕西省猪瘟病毒E2基因主要抗原区变异情况,为猪瘟防控提供依据。用巢式PCR方法对猪瘟疫苗和采自陕西省部分地区的疑似猪瘟病料进行E2基因主要抗原区扩增并测序,将测序结果与HCLV疫苗株和Shimen株E2基因进行序列比对分析。结果表明,23株流行毒株之间的核苷酸同源性在86.0%～100%,氨基酸同源性在87.8%～100%;与HCLV株相比,核苷酸同源性在77.2%～81.2%,氨基酸同源性为78.9%～88.9%;与经典强毒Shimen株核苷酸同源性为77.2%～80.9%,氨基酸同源性为80.0%～90.0%。2株疫苗毒E2基因和HCLV株相比较,核苷酸同源性为93.4%,氨基酸同源性为90.0%;和Shimen株核苷酸同源性为94.1%,氨基酸同源性为92.2%。疫苗和经典HCLV株E2主要抗原区相比,在所测定的91个氨基酸位点中有9个位点发生了变异,流行毒株相对于HCLV株有12个氨基酸位点发生了变异。流行毒株E2基因主要抗原区发生了较明显的变异,疫苗E2基因主要抗原区和HCLV株相比发生了较为明显的变异。疫苗和流行毒株只有3个变异位点相同,提示疫苗和流行毒的变异趋势不一致。
To get useful data for the prevention and control of classical swine fever,the main antigen variation of E2 gene of classical swine fever virus in Shaanxi Province were analyzed by nested RT-PCR amplified.The main antigen of E2 gene of the vaccines and parts of samples from the suspected classical swine fever from Shaanxi Province was sequenced,which was compared with HCLV vaccine strains and Shimen strain E2 gene sequence.The results showed the nucleotide and amino acid homology of 23 pandemic strains are 86.0%～100% and 87.8%～100%,respectively.The nucleotide and amino acid homology between the pandemic strains and HCLV strain are 77.2%～81.2% and 78.9%～88.9%,respectively.And the nucleotide and amino acid homology between the pandemic strains and Shimen strains are 77.2%～80.9% and 80.0%～90.0%,respectively.Compared E2 gene with HCLV,the nucleotide and amino acid homology are 90.0% and 93.4%,respectively.And nucleotide and amino acid homology are 94.1% and 92.2% between Shimen strains and E2 gene,respectively.Compared the main antigen of E2,9 of 91 amino acid sites in HCLV mutated,and compared with HCLV,12 amino acid sites in pandemic strains mutated.Prevalent virulent strains E2 gene mutate obviously,E2 gene in vaccine occurred obviously variation compared to HCLV.Vaccine and epidemic strains have only three same variable sites,which suggested that vaccine and epidemic strains have different variation trend.
出处
《上海交通大学学报（农业科学版）》
2011年第6期24-28,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
咸阳市科技计划项目(211K04-12)
关键词
猪瘟
E2基因
序列分析
classical swine fever
E2 gene
cloning
sequence analysis
