摘要
目的:为中药壁虎质量标准的制订与资源开发提供科学的实验依据及可靠的实验方法。方法:提取不同产地的无蹼壁虎药材不同部位(壁虎体与壁虎尾)的水溶性蛋白质,并对其进行含量测定和聚丙烯酰胺凝胶电泳,比较壁虎药材蛋白质含量和组成的产地差异和部位差异。结果:不同产地壁虎药材的蛋白质含量不同,同一产地壁虎药材的不同部位蛋白质含量也存在差异。蛋白质电泳图谱显示,除共有条带外,同一提取部位的蛋白条带的分布位置和数量随产地存在一定差异。结论:考马斯亮蓝染色法简便、快速,标准曲线线性关系良好,可以用来进行蛋白质含量的测定。聚丙烯酰胺凝胶电泳实验快速、准确、重复性好,得到的蛋白质电泳指纹图谱可作为壁虎药材质量评价和资源开发的重要依据之一。
Objective：To provide scientific experimental basis and reliable methods for establishing the quality standards and exploiting resources of the traditional Chinese medicine Gekko swinhonis.Method：To compare the habitat and organ difference of protein,the water-soluble protein of different parts（body and tail） of G.swinhonis from different habitats was extracted.The protein contents were determined and the experiments of PAGE were conducted.Result：The protein content of G.swinhonis varied with the habitat,and it also varied with the part from the same habitat.The PAGE electrophoretogram showed that except for the common bands,there was difference in the distribution and number of the protein bands among different samples.Conclusion：The method of coomassie brilliant blue staining is simple and fast with good correlation coefficient,and can be used to determine the protein content of medicinal materials.The experiment of PAGE is rapid,accurate and reproducible,and the protein electrophoresis fingerprint can serve as one of the important bases for the quality evaluation and resources exploitation of G.swinhonis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第1期69-72,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
<山东省中药材标准>2012版标准研究
关键词
无蹼壁虎
蛋白质
聚丙烯酰胺凝胶电泳
含量测定
Gekko swinhonis
protein
polyacrylamide gel electrophoresis
content determination
