摘要
目的:采用定性均匀设计分析补气生血方剂对失血性血虚模型小鼠的外周血细胞的作用,寻求发挥最佳药效的配伍。方法:将因素A补气药的12个水平:人参、西洋参、党参、太子参、黄芪、白术、山药、白扁豆、甘草、大枣、刺五加、灵芝与因素B补血药的6个水平:当归、熟地黄、何首乌、白芍、阿胶、龙眼肉,按定性均匀设计U24(242)表组成24个补气生血方剂。小鼠眼眶放血0.5 mL/只,复制失血性血虚模型,测定造模后第1,4,7,10天小鼠外周血细胞值,观察各补气生血方剂对血虚小鼠白细胞计数(WBC),红细胞计数(RBC),血红蛋白(HGB),红细胞比容(HCT),血小板计数(PLT)的影响,找出反映药效变化最明显的时间点。用主成分分析法分析最明显时间点的WBC,RBC,HGB,HCT,PLT数值及其相互关系,得出主因子。以主因子为指标,建立定性均匀设计中虚拟变量的多元线性回归方程,找出最佳药效配伍。结果:RBC,HGB,HCT变化最明显的时间点为造模后第7天,反映药效的主因子为RBC,HGB,HCT。以RBC,HGB,HCT第7天的值为指标,建立了正确的多元线性回归方程,得出最佳药效配伍为人参当归。结论:对于失血性贫血模型小鼠,人参当归配伍为补气生血方剂中的最佳药效配伍,定性均匀设计法可用于中医方剂配伍研究。
Objective：To analysis the effects of invigorating Qi and promoting blood on hemorrhagic blood deficient in mouse by using qualitative analysis of uniform design and to find out the optimum prescription composition.Method：Twelve supplement Qi medicines,such as Ginseng Radix Et Rhizoma,Panacis Quinquefolii Radix Et Rhizoma,Codonopsis Radix,Pseudostellariae Radix,Astragali Radix,Atractylodis Macrocephalae Rhizoma,Dioscoreas Rhizoma,Lablab Semen Album,Glycyrrhiae Radix,Jujube Fructus,Acanthopanacisi Senticosi Radix Et Rhizoma Seu Caulis,Ganoderma,and 6 supplement blood medicines,such as Angelicae Sinensis Radix,Rehmanniae Radix,Polygoni Multiflori Radix,Paeoniae Radix Alba,Asini Corii Colla,Longan Arillus,were composed of 24 supplement Qi and engendering blood prescriptions according to U^24（2^42） qualitative analysis of uniform design.The hemorrhagic blood deficient mouse model was obtained with 0.5 mL orbital bleeding.Peripheral blood cells were counted after 1 d,4 d,7 d,10 d modeling to find the best diversify time of potency.The content of RBC,HGB,HCT,WBC,PLT of the obvious effective time and the relationship between effect with administration time were analyzed using principal component analysis method to find out the major cause.Multiple linear regression with dummy variable was set up to find out the best prescription composition.Result：The best diversify time of potency was on 7 d.RBC,HGB,HCT were the major cause indexes.The multiple linear regression were set up and the composition of Ginseng Radix and Astragali Sinensis Radix appears to be the best compatibility for the efficacy.Conclusion：The composition of Ginseng Radix and Astragali sinensis Radix is the best compatibility for blood loss anemia mouse.Qualitative analysis of uniform design is feasible to be used in the compatibility study.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第1期129-134,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30860350)
关键词
定性均匀设计
虚拟变量
主成分分析
失血性血虚
补气生血
qualitative analysis of uniform design
dummy variable
principal component analysis
hemorrhagic blood deficient
reinforcing qi and engendering blood
