摘要
目的探讨脂多糖诱导大鼠肝细胞的损害特点,并探讨三七皂甙Rg1抗肝细胞损害的保护作用。方法用胶原酶灌流法分离肝细胞。脂多糖诱导大鼠肝细胞损害,透射电镜观察肝细胞形态学的变化,DNA凝胶电泳观察其生化变化,用单细胞凝胶电泳法(SCGE)进一步探讨肝细胞损害的特点,并探讨三七皂甙Rg1对肝细胞损害的保护作用。结果由脂多糖诱导的肝细胞在形态上表现出染色质凝聚、凋亡小体形成等凋亡特征,生化方面表现出典型的DNA梯度,此过程可被凋亡抑制剂Quinaceine抑制。用SCGE法检测出,肝细胞凋亡和死亡数量与脂多糖的诱导时间有明显的相关性。在此基础上,观察到三七皂甙Rg1可抑制肝细胞凋亡和坏死,其抑制效果与Quinaceine无明显差异。结论三七皂甙Rg1可显著抑制肝细胞凋亡和坏死,是防治肝细胞损害的良好药物。
Objective To investigate the effect of liposaccharide （LPS） on hepatocyte damage and find out effective hepatoprotectants by means of single cell gel electrophoresis. Methods The hepatocytes were isolated in the way of liver perfusion with collagenase. Transmission electron microscope technique was used to observe the morphological change of hepatocyte. The biochemical changes were analyzed by the DNA agarose gel electrophoresis. The SCGE was used to study hepatic damage and find out effective hepatoprotectants. Results The hepatocytes incubated with LPS exhibited the condensation of nuclear chromatin and formed apoptotic bodies. The DNA fragment was demonstrated in biochemistry, but it was inhibitory by Quinacrine. The rate of hepatic apoptosis and necrosis has a good conelationship with LPS-treated time. Panax notoginseng saponins Rgl can inhibit both of them. The inhibited effect had no difference with Quinacrine. Conclutions Panax notoginseng saponins Rgl can inhibit hepatic apoptosis and necrosis remarkebly, It has a good protective effect on damage of rat hepatocytes.
出处
《环球中医药》
CAS
2012年第1期19-22,共4页
Global Traditional Chinese Medicine
关键词
脂多糖
单细胞凝胶电泳
三七皂甙RG1
肝细胞损害
Liposaccharide
Single cell gel electrophoresis
Panax notoginseng saponins Rgl
Liver damage
