摘要
目的:观察大鼠脑缺血再灌注后脑内脑源性神经营养因子(BDNF)mRNA和蛋白的表达变化以及清热化瘀方对其影响。方法:采用线栓法制作局灶性脑缺血大鼠(MCAO)模型,用原位杂交法和免疫组化法观察脑缺血后3、6、12、24h及3、7d共6个时间点BDNF mRNA及其蛋白的表达变化,以TUNEL法检测神经细胞凋亡。结果:缺血半暗带BDNF mRNA及其蛋白的表达均于缺血再灌注后3h开始明显上升,6h表达继续增强,12h达高峰,3d后表达开始减弱(P<0.05,P<0.01);神经细胞凋亡于缺血再灌注后3h开始明显上升,24h达高峰,3d后表达开始减弱(P<0.05,P<0.01)。清热化瘀方治疗后可以上调BDNF的表达,减轻神经细胞凋亡(P<0.05,P<0.01)。结论:清热化瘀方可促进脑缺血后BDNF的表达,保护神经元。
Objective： To observe the mRNA and protein levels of brain-derived neurotrophic factor（BDNF） in rats after focal cerebral ischemia/reperfusion（I/R） and effect of Qingre Huayu Prescription（QRHYP） on them.Methods： The I/R rat models were established by middle cerebral artery occlusion（MCAO） method to observe the expression variation of BDNF mRNA and protein at 3,6,12h and 1,4,7days after I/R by in situ hybridization and immunochemistry method,and the number of apoptosis neuron observed by TUNEL method.Results： The expressions of BDNF mRNA and protein in the penumbra region increased at 3h after reperfusion,and reached the peak after 12h,then decreased continuously after 3days（P0.05,P0.01）.The apoptosis neuron increasedsignificantly at 3h after reperfusion,and reached the peak after 24h,then decreased continuously but were yet higher than the norma1 1evel after 7days（P0.05,P0.01）.QRHYP treatment could increase the expressions of BDNF and decrease apoptosis neuron（P0.05,P0.01）.Conclusions： QRHYP could decrease neuronal apoptosis through upregulating the expression of BDNF after I/R in rats.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第1期159-161,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
广西自然科学基金(No.0832171)~~
