摘要
目的:建立九里香中橙皮内酯和脱水长叶九里香内酯含量测定方法,并测定其在广西不同产地及药材中不同部位的含量。方法:采用高效液相色谱法,使用Agilent Esclipe XDB-C18色谱柱(150mm×4.6mm,5μm),流动相为甲醇-水(57:43),流速0.7mL/min,检测波长332nm,柱温25℃。结果:橙皮内酯进样量在0.092-1.104μg,脱水长叶九里香内酯进样量在0.14-1.68μg范围内,与峰面积呈良好的线性关系,相关系数r均为0.9999(n=5);橙皮内酯与脱水长叶九里香内酯的平均回收率(n=6)分别为98.25%和98.30%,RSD分别为0.76%和1.19%。结论:本法结果准确,操作简便,重现性好,可为九里香药材的质量控制提供方法。
Objective： To develop a method for the determination of meranzin and phebalosin for analyzing the content in Murraya exotica L.in different origins and different medicinal portions.Methods： The separation was performed on Agilent Esclipe XDB-C18 column（150mm×4.6mm,5μm）,using a mixture of 57% methanol and 43% water as the mobile phase at a flow rate of 0.7mL/min at 25℃,the wavelength for measurement was 332nm.Results： The calibration curves were linear in the range of 0.092-1.104μg for meranzin and 0.14-1.68μg for phebalosin,and the both correlation coefficients（n=5） were 0.9999;the average recovery（n=6） for meranzin and phebalosin were 98.25%（RSD=0.76%） and 98.30%（RSD=1.19%）.Conclusion： The method is accurate,reliable and reproducible to determine meranzin and phebalosin in Murraya exotica L..
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第1期169-171,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
广西科技厅科技攻关项目(No.桂科攻0815005-1-22)~~
