摘要
参考黑线仓鼠(Cricetulus barabensis)近缘种PPARβ的cDNA序列设计引物,采用RT-PCR法克隆得到黑线仓鼠PPARβ部分序列,并翻译成氨基酸.利用该结果与其他物种核苷酸及氨基酸序列进行相似性比较、构建系统进化树、一级结构分析、二级结构和空间结构预测.结果表明:得到的480 bp PPARβ序列包括部分外显子4,全部外显子5、6及部分外显子7;共编码159个氨基酸;除人之外,黑线仓鼠PPARβ基因与其他物种的核苷酸相似性均在88%-94%之间,氨基酸相似性均在90%以上;构建进化树得出,黑线仓鼠与鼠类亲缘关系最近,与人的亲缘关系最远;疏水性分析、二级结构预显示该编码区亲水性较强;二级结构、空间结构预测显示该序列含α螺旋较多;空间结构预测看出该段区域含两个典型的锌指结构.
Partial cDNA sequence of the PPARfl was cloned by primer and RT-PCR method. The cDNA se- quence was translated into amino acid sequence. Using the results, the similar comparison with other species and the amino acid sequence of nucleotides was plone, the phylogenetic tree was built, the primary structure, secondary structure and spatial structure prediction were andysized. The results show that： the 480 bp PPARβ sequence includes partial exon 4, the whole exon 5, the whole exon 6 and partial exon 7 ; Code of 159 amino acids; except the human, the nucleotide identities between Cricetulus barabensis PPARβ genes and other species are 88% and 94% , amino acids are above 90% ; phylogenetie tree shows that the relationship between C. barabensis and rodents is of the closest, and people＇s relationships outside is of the farthest ; hydrophobic analysis, secondary structure show that the hydrophilic coding areas is stronger; secondary structure, space structure prediction show the sequence contains more （x-helix; space structure prediction shows this zone containing two typical zinc finger structure.
出处
《曲阜师范大学学报（自然科学版）》
CAS
2012年第1期85-90,共6页
Journal of Qufu Normal University(Natural Science)
基金
国家重点基础研究发展计划项目(2007CB109104)
国家自然科学基金(31070332)
曲阜师范大学"十一五"计划省级重点建设项目
山东省高校科技计划项目(J09LC18)资助
山东省自然科学基金ZR2010CL013
山东省中青年科学家科研奖励基金(BS2010NY012)
关键词
黑线仓鼠
PPARβ
克隆
序列结构
系统进化
空间结构
锌指结构
Cricetulus barabensis
PPARβ
cloning
sequential structure
phylogenetie evolution
space structure
zinc finger structure
