摘要
目的评价18F标记脱氧葡萄糖-正电子发射扫描成像（18F-FDG-PET）在小鼠重症急性胰腺炎早期诊断和疗效评价中的应用价值。方法采用连续7次腹内注射雨蛙素（每次间隔1h）及脂多糖制作小鼠重症急性胰腺炎模型，57只雌性ICR小鼠随机分为硼替佐米（PS-341）治疗组（注射脂多糖前0．5h腹内注射0．5mg／kgPS-341），模型对照组[注射脂多糖前0．5h腹内注射50％二甲基亚砜（DMSO）]，空白对照组（生理盐水制模）。首次注射雨蛙素后8h将小鼠处死，每组3只行PET胰腺扫描，8只取胰腺组织测髓过氧化物酶（MPO），另外8只光学显微镜下观察小鼠胰腺的病理形态。结果正常对照组PET扫描时胰腺不显影，与模型对照组比较，治疗组小鼠胰腺18F-FDG的吸收率（5．27±0．35，3．48±0．49）、胰腺MPO活性（3．46±0．28、1．82±0．54）均显著降低，两者之间差异有统计学意义（P〈0．05）；胰腺组织的病理学也得到明显改善，进一步验证了18F-FDG—PET在重症急性胰腺炎诊断和疗效评价中的应用价值。结论18F．FDG-PET能够动态监测重症急性胰腺炎的发展和转归，评价治疗效果。
Objective To investigate the potential value of （F-18）-fluoro-deoxy-D-glueose posi- tron emission tomography （18F-FDG-PET） in the early diagnosis and evaluating the effects of interventions for severe acute pancreatitis in mice. Methods Fifty-seven female ICR mice weighing （ 20±1 ） g were used in this study. There were 19 mice in each group. The model severe acute pancreatitis was induced by the mothod reported previously. Thirty min before the administration of lipopolysaccharide, mice in the PS- 341 treatment group were treated intraperitoneally with 0. 5 mg/kg PS-341, and those in the control groups were treated with an equal volume of vehicle. Eight h after the first cerulein injection, mice were anesthetized with 50 mg/kg pentobarhital. Three mice were used for the 18F-FDG-PET examination, 8 mice for the detection of pancreatic myeloperoxidase （MPO） activity, and the rest 8 mice for pancreatic morphological changes in histological sections. Results The pancreatic appearance could not be seen in the PET image in normal group; while in those mice with severe acute pancreatitis, the 18F-FDG uptake was significantly increased （5.27±0. 35）. PS-341 treatment significantly reduced the uptake of 18F-FDG within the pancreas （3.48±0. 49）. MPO assay also demonstrated that induction of severe acute pancreatitis elevated the activity of MPO （3. 46±0. 28）, and PS-341 treatment significantly reduced MPO activity （ 1.82 ±0. 54）. This result was in accordance with that of the positron emission tomography. Conclusion 18F-FDG-Positron emission tomography could be a sensitive and promising means in evaluating the therapeutic effect and adjusting medical interventions in pancreatitis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第1期30-32,F0003,共4页
Chinese Journal of Experimental Surgery
基金
浙江省科技厅资助项目（2006C33077）
