摘要
目的:建立人卵巢癌裸鼠腹腔及皮下移植瘤模型,比较2种模型的生物学特性。方法:采用细胞悬液注射法将人上皮性卵巢癌细胞A2780/DDP注射于裸鼠皮下或腹腔,建立人卵巢癌裸鼠移植瘤模型,观察模型动物肿瘤的生长规律及组织学特性,流式细胞术分析细胞周期分布,免疫组化检测促黄体激素释放激素受体(Luteinzing-hormone releasing hormone receptor,LHRH-R)表达情况。结果:裸鼠腹腔及皮下移植瘤模型的成瘤率均为100%,2组荷瘤鼠中位生存时间分别为(30.000±1.095)d和(41.000±0.894)d,2组移植瘤细胞周期分布与原代细胞周期分布相仿,且2种模型的组织形态学和超微结构相似,免疫组化显示均表达LHRH-R。结论:人卵巢癌细胞株A2780/DDP裸鼠皮下和腹腔移植瘤模型易于建立,腹腔移植瘤模型能更好地模拟人卵巢癌腹腔播散的生物学行为,是研究卵巢癌治疗较理想的动物模型。
Objective：To establish intraperitoneal（IP） injection model and subcutaneous（SC） injection model of human ovarian carcinoma in nude mice and compare their biological characteristics.Methods：The cell suspension derived from A2780/DDP were injected intraperitoneally or subcutaneously in nude mice to establish human epithelial ovarian carcinoma models,and their growth behavior and biological characteristics were observed.Distribution of cell cycles were analyzed by flow cytometry（FCM）.Expression of LHRH-R were investigated by immunohistochemistry.Results：The human ovarian carcinoma models were established successfully.The success rate of transplantation in both groups were 100%.The median survival time lengths in IP group and SC group were 30.000±1.095 days and 41.000±0.894 days,respectively.FCM analysis showed the distribution of cell cycles of xenografted tumors in two groups were the same as those of A2780/DDP cells.Histology and electron microscopy showed the same characteristics and morphological properties in the two groups,and immunohistochemistry staining of LHRH-R were both positive.Conclusion：Human ovarian carcinoma models in nude mice are easy to establish.And the IP model may be an ideal model for treatment research of ovarian carcinoma as it simulates the intraperitoneally dessiminating behavior of human ovarian carcinoma in the patients.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2011年第11期1343-1346,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目(编号:30801228)
重庆市自然科学基金资助项目(编号:CSTC
2008BB5405)
重庆市卫生局资助项目(编号:07-2-098)
关键词
上皮性卵巢癌
动物模型
裸鼠
促黄体激素释放激素
epithelial ovarian carcinoma
animal model
nude mice
luteinzing-hormone releasing hormone（LHRH）
