摘要
目的探讨部分免疫活性细胞与自身免疫性溶血性贫血(autoimmune hemolytic anemia,AIHA)发病的关系及输血影响。方法将67例AIHA患者作为观察组,其中28例输血患者为A组,70例非AIHA其他贫血患者作为对照组,其中30例输血患者为B组。采用流式细胞术测定观察组部分免疫细胞活性水平,并分别与对照组、正常健康组测定值比较,同时对A、B两组患者进行输血前后部分免疫指标比较。结果①观察组用药前外周血CD3^+、CD4^+、CD4^+/CD8^+、NK细胞活性水平均显著低于正常组(P<0.05);观察组CD19^+指标上升明显高于对照组,而对照组CD19^+指标又明显高于正常组,(P<0.05)均有统计学意义。②联合检测AIHA患者T细胞亚群、B细胞、NK细胞的阳性表达率高于单项阳性表达率。③A组输血后外周血CD3^+、CD4^+、CD4^+/CD8^+比值上升、CD19^+细胞表达下降较治疗前有明显差异。结论 AIHA患者存在外周血部分免疫活性细胞表达异常,输血能改善AIHA患者的细胞免疫平衡调节功能。
Objective To investigate the cellular immunity autoimmune hemolytic anemia（AIHA） and the effect of transfusion.Methods Sixty seven AIHA patients,including 28 receiving blood transfusion （group A） and 70 non-AIHA patients,including 30 receiving transfusion（group B） were enrolled in the study.The levels of CD3~＋,CD4~＋,CD4~＋/CD8~＋,CD19~＋ were determined by flow cytometry（PCM）. Those immune indicators were compared between ALHA and non-AIHA patients;before and after transfusion.Results The levels of the peripheral blood of T cell subgroup and NK cell activities in AIHA patients were significantly lower than those of non-AIHA group（P 0.05）.The abnormal rate of combined tests of T cell subgroup,B cells and NK cells in AIHA patients was higher than that of single tests. The expression of CD3~＋,CD4~＋,and CD4~＋CD8~＋ in group A increased and CD19~＋ declined after transfusion. Conclusion Blood transfusion can improve the abnormal cell immunity function in AMA patients.
出处
《同济大学学报（医学版）》
CAS
2011年第6期63-66,共4页
Journal of Tongji University(Medical Science)
基金
上海市卫生局科研项目(WSJ1102)
关键词
免疫性溶血性贫血
免疫细胞
活性
输血
autoimmune hemolytic anemia
immunocyte
active
transfusion
