摘要
目的:探讨Neuropilin-1(NRP-1)mRNA在繁殖适龄期小鼠动情周期间以及不同周龄阶段小鼠卵巢中的表达。方法:选用繁殖适龄期不同动情周期小鼠以及不同周龄阶段小鼠,通过实时定量聚合酶链反应检测其卵巢中NRP-1 mRNA的表达。结果:NRP-1在小鼠不同动情周期卵巢中的表达在mRNA水平差异无显著性(P>0.05)。比较其mRNA在不同周龄阶段小鼠卵巢的中表达显示,近绝育期32周组表达水平显著高于其他组:为3周组的2.94倍(P<0.01),6周组的4.92倍(P<0.01),11周组的2.29倍(P<0.01)。而其他各组间比较差异无统计学意义(P>0.05)。结论:NRP-1 mRNA在小鼠卵巢中的表达不随动情周期而改变,且随着卵巢生理功能的下降而显著升高,提示NRP-1与卵巢衰老进程有关。
Objective To explore the expression of neuropilin-1 （NRP-1） mRNA in mouse ovaries during estrous cycle and different age stages. Method Real-time polymerase chain reaction was used to detect the expression of NRP-1 mRNA in mouse ovaries during estrous cycle and different age stages. Results There was no difference of NRP-1 expression in ovary among estrous cycles（P 〉 0.05 ）. However, there were significant differences among the different age stages. The NRP-1 mRNA in ovary in penuriously functional stage （32 weeks）was significantly higher than that in other stages, which was 2.94 times in 3 weeks （P 〈 0.01 ）, 4.92 times in 6 weeks （P 〈 0.01）, and 2.29 times in 11 weeks （P 〈 0.01）. Conclusion The level of NRP-1 mRNA in mouse ovary didnJt change among the estrous cycles, while it significantly increase when ovarian function decrease, which indicate that NRP-1 play an important role in the process of ovarian aging.
出处
《实用医学杂志》
CAS
北大核心
2012年第1期45-48,共4页
The Journal of Practical Medicine
基金
卫生部公益性行业科研专项项目(编号:200802159)
教育部高等学校博士点科研基金项目(编号:200804870009)
国家自然科学基金项目(编号:30973148)
