摘要
通过对苏云金芽孢杆菌以色列亚种Bti L-10菌株进行培养基的改良优化得到一种理化性质好、利于后处理的配方。200 L罐发酵效价达395 ITU/μL;通过后处理工艺的优化,得到一种制备1 200 ITU/mgBti浓悬浮剂路线,即发酵液2次离心并高倍浓缩得到菌浆,添加各种助剂经砂磨机研磨,得到Bti浓悬浮剂产品效价达到1 245 ITU/mg。
The culture medium for highly toxic Bacillus thuringiensis subsp.israelensis L-10 strain was obtained by orthogonal tests of nitrogen source.The formula with good physicochemical properties was conducive to the post-treatment.In the pilot fermentation in 200 L fermentors,the potency of the fermentation broth was 395 ITU/μL.After the optimization of post-treatment,the optimal route of 1 200 ITU/mg Bti suspension concentrate was developed.It was prepared by using fermentation broth to the secondary centrifugation and high-powered concentration,then adding additive and grinding with sand mill.Its titer reached 1 245 ITU/mg against mosquito larva.
出处
《湖北农业科学》
北大核心
2011年第24期5113-5115,共3页
Hubei Agricultural Sciences
基金
国家科技支撑计划项目(2011BA40B00)
湖北省农业科技创新中心资助项目(2007-620-001-03)
关键词
苏云金芽孢杆菌以色列亚种
浓悬浮剂
制备工艺
Bacillus thuringiensis subsp.israelensis
suspension concentrate
optimal route
