摘要
以刺槐(Robinia pseudoacacia L.)、红花刺槐(R.pseudoacacia var.decaisneana Carr.)、四倍体刺槐(Tetraploid R.pseudoacacia L.)的花粉为材料,研究了蔗糖、硼酸和硝酸钙对3种刺槐花粉萌发率及花粉管伸长生长的影响。结果表明:蔗糖、硼酸和硝酸钙对3种刺槐的花粉萌发和花粉管的伸长有明显促进作用,且影响程度因种而异。刺槐和红花刺槐的平均花粉萌发率明显高于四倍体刺槐。不同种刺槐的花粉萌发最适培养基不同;同种刺槐,不同花粉采集期的最适萌发培养基也不尽相同,并得出了每种花粉各自的最适萌发培养基。
An experiment was conducted to study the effects of sucrose, boric acid and calcium nitrate on pollen germination and pollen tube growth of Robinia pseudoacacia var. decaisneana Carr. , R. pseudoacacia L. , and Tetraploid R. pseudoacac/a. Results showed that sucrose, boric acid and calcium nitrate could obviously promote pollen germination and pollen tube elongation, and the influence degrees of the three factors were various between different species of black locusts. The average pollen germination rates of R. pseudoacacia and R. pseudoacacia var. decaisneana were significantly higher than that of Tetraploid R. pseudoacacia. The optimum culture media for pollen germination were different for different species of black locusts. Moreover, the optimum culture media for the germination of pollens collected at different developmental stages were also various for the same species. The optimum culture media for pollen germination were obtained for each species.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2012年第1期1-5,共5页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(30640036)
国家"十一五"科技支撑项目(2006BAD01A1601)
林业公益性行业科研专项(201104013)
关键词
刺槐
花粉
体外萌发
培养基
影响因子
Black locust
Pollens
in vitro germination
Culture media
Influence factors
