摘要
目的:分离纯化白茅根多糖,建立白茅根多糖相对分子质量的测定方法,并通过高效液相色谱法测定其单糖组成和摩尔比。方法:通过多次醇沉的方法对白茅根多糖进行分离纯化,得到白茅根多糖IC1;采用高效液相色谱法,以右旋糖酐为标样,绘制多糖分子量的标准曲线,测定白茅根多糖IC1的相对分子质量;通过酸水解,使IC1水解成单糖,分析这些单糖的组成及其摩尔比。结果:经测定,白茅根多糖IC1的相对分子质量为8 292.2;白茅根多糖IC1由鼠李糖,木糖,果糖,甘露糖,葡萄糖5种单糖组成,其摩尔比为1∶11.45∶1.26∶1.02∶59.23。结论:多次醇沉的方法对白茅根多糖进行分离,效果良好,操作简便。高效液相法测定白茅根多糖分子质量及单糖组成灵敏度高,重复性好。
Objective：To study on isolation the polysaccharide from Imperata cylindrica and to establish a method for the determination of the molecular weight of the polysaccharides in Imperata cylindrica,and to measure its composition and mole ratio through HPLC.Method：The polysaccharide in Imperata cylindrica was Isolated and purified through the way of the staged alcohol deposition.Dextran was used as standard sample to draw the molecular weight standard curve,and the molecular weight of Imperata cylindrica polysaccharides was determined by HPLC,the polysaccharide was hydroyzed by acid to analyze monosaccharide composition and the mole ratio by HPLC.Result：The molecular weight of Imperata cylindrica polysaccharide was 8 292.2.According to HPLC IC1 was composed of Rhamnose,Xylose,Fructose,Mannose and Glucose,the mole ratio of these monosaccharides was 1∶11.45∶1.26∶1.02∶59.23.Conclusion：This method is simple accurate and sensitive.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第2期80-82,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部重大创制候选药物项目(2009ZX09103-354)
关键词
白茅根
多糖
分离纯化
高效液相色谱法
相对分子质量
单糖组成
摩尔比
Imperata cylindrica
polysaccharide
isolation and purification
HPLC
molecular weight
monosaccharide composition
mole ratio
