摘要
目的:用高效液相色谱法测定齐酞酸钠的含量及其有关物质。方法:采用Eclipse XDB-C18色谱柱(4.6 mm×150mm,5μm),以甲醇-乙腈-0.1%磷酸水溶液(80∶10∶10)为流动相,流速1.0 mL.min-1,柱温35℃,检测波长210 nm。结果:齐酞酸钠与其相邻杂质峰能完全分离,齐酞酸钠在0.052 5～0.997 5μg进样量与峰面积呈良好线性关系(r=0.999 9),最低检出限为3 ng;精密度良好(RSD 0.38%),平均回收率为99.53%(n=9)。结论:该方法简便、准确、专属性好、灵敏度高,可用于齐酞酸钠的含量及有关物质测定。
Objective：To determine the content and the related substances of oleanolic acid 3β-phthalic monoester disodium salt（OAPES） by HPLC.Method： The separation was performed on Eclipse XDB-C18 column（4.6 mm×150 mm,5 μm）,column temperature at 35 ℃ with mobile phase of methanol-acetonitrile-0.1% phosphoric acid solution（80∶10∶10）.The flow rate was set at 1.0 mL·min-1 with detection at 210 nm.Result： There was a good linear relationship within the range of 0.052 5-0.997 5 μg（r=0.999 9）,the least detective range is 3 ng.The precision in a day is good（RSD 0.38%）.The average recovery was 99.53%（n=9）.Conclusion： The method is simple,accurate,specific and sensitiveness for the determination OAPES content and its related substances.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第2期89-91,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省科技厅攻关项目(黔科合字[1998]9号)
关键词
齐酞酸钠
含量
有关物质
高效液相色谱法
oleanolic acid 3β-phthalic monoester disodium salt
content
related substances
HPLC
