摘要
目的:观察檀香不同提取物对小肠推进和胃排空的影响,探讨其作用机制。方法:小鼠随即分为空白对照组,各提取部位组。各给药组ig相应药物,给药剂量按生药量计为1.9 g.kg-1,空白对照组ig等体积蒸馏水。每日1次,连续ig 3d。采用酚红排空法,以正常、新斯的明、阿托品负荷为实验模型,观察檀香不同提取物对小鼠小肠推进和胃排空的影响。结果:檀香水提液、醇提液、挥发油、水提液乙醚萃取部位对正常小鼠胃排空有抑制作用(P<0.05),表现为胃残留率增大。檀香水煎液、醇提液、水提液乙醚萃取部位阿托品造成的小鼠胃排空抑制模型有拮抗作用(P<0.05),表现为残留率减小,檀香水煎液对阿托品造成的小鼠小肠推进抑制模型有拮抗作用(P<0.05),表现为推进率增大。檀香水煎液、醇提液、挥发油、醇提液乙酸乙酯萃取部位对新斯的明造成的小鼠胃排空亢进模型有拮抗作用(P<0.05),表现为残留率增大,檀香水煎液、醇提液、挥发油、水提液乙醚萃取部位、醇提液乙醚萃取部位、醇提液乙酸乙酯萃取部位对新斯的明造成的小鼠小肠推进亢进模型有拮抗作用(P<0.05),表现为推进率减小。檀香水提液部位、醇提液部位、挥发油部位和醇提液乙醚部位对新斯的明造成的小鼠胃排空亢进模型有拮抗作用(P<0.05),表现为排空率增大。结论:檀香对小鼠小肠推进和胃排空有双向调节作用,檀香水煎液、醇提液对阿托品造成的肠松弛模型及新斯的明造成的肠痉挛模型均有明显的拮抗作用,檀香挥发油在新斯的明造成的肠痉挛模型中表现出明显的拮抗作用,故可认为檀香水煎液、醇提液、挥发油是檀香"行气"作用的有效部位。
Objective：To observe effects and mechanism of the Santali Albi Lignum different extracts on small intestine propulsion and gastric emptying.Method： Mice were randomly divided into control group and groups of extracted parts.The treatment groups take corresponding exreacts with dose of 1.9 g·kg-1,once a day for 3 days.Phenol red emptying method was used,normal,neostigmine and atropine load mice were used for experimental models.Result： Santali Albi Lignum water liquid,ethanol-extraction liquid,naphtha,water liquid ether extract part of the gastric inhibited emptying normal mice（P0.05）,the stomach and retention rate increased.Santali Albi Lignum water fluid,liquid water extract-alcohol ethanol-extraction,liquid ether extract part inhibited emptying in atropine gastric inhibition models（P0.05）,the residual rate decreased,and Santali Albi Lignum water decoction inhibited small intestine movement in atropine mice models（P0.05）,promoting rate increased.The decoction of Santali Albi Lignum,ethanol-extraction liquid,naphtha,ethanol-extraction liquid ethyl acetate extract inhibited gastric emptying in neostigmine induced mice model（P0.05）.The performance for the retention rate increased,the decoction of Santali Albi Lignum,ethanol-extraction liquid,naphtha,water liquid ether extract parts and ethanol-extraction liquid ether extract parts and ethanol-extraction liquid ethyl acetate extract inhibited small intestine propulsion in model mice induced by neostigmine（P0.05）,promoting rate reduced.Santali Albi Lignum water liquid part,ethanol-extraction liquid part,naphtha parts and ethanol-extraction liquid petroleum ether parts and ethanol-extraction liquid ether inhibited mice induced gastric emptying in model mice induced by neostigmine（P0.05）,emptying rate increased.Conclusion：Santali Albi Lignum on small intestine propulsion and gastric emptying have two-wayregulation function,Santali Albi Lignum water fluid,ethanol-extraction liquid in atropine and neostigmine models have significantl inhibition Santali Albi Lignum,and volatile model experiments showed obvious inhibitory effect on intestines convulsion,therefoer decoction of ethanol-extraction,liquid,naphtha is the effective parts.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第2期139-143,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部"973"项目(2007CB512603)
国家重点基础研究发展计划项目(2007CB512600)
湖南省科技计划项目(2007WK3033)
湖南省中药重点学科
关键词
檀香
小肠推进
胃排空
吸光度
新斯的明
阿托品
Santali Albi Lignum
small intestine
gastric emptying
absorbance
neostigmine
atropine
